Myosin Light Chain 2/MLC-2V Polyclonal antibody 55462-1-AP proteintech
$149.00
In stock
SKU
55462-1-AP
| Catalog Number | 55462-1-AP |
| Synonyms | MLC 2v, MYL2, MLC 2, MLC2, MLC-2 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, FC (Intra), IP, ELISA |
| Applications | WB, IHC, FC (Intra), IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | rat heart tissue |
| Tested Applications — Positive IP detected in | mouse brain tissue |
| Tested Applications — Positive IHC detected in | mouse brain tissue, human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive FC (Intra) detected in | C2C12 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:20000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | rat heart tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | C2C12 cells |
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | myosin, light chain 2, regulatory, cardiac, slow |
| Calculated Molecular Weight | 19 kDa |
| Observed Molecular Weight | 18-20 kDa |
| GenBank Accession Number | NM_000432 |
| Gene Symbol | Myosin Light Chain 2 |
| Gene ID (NCBI) | 4633 |
| RRID | AB_2881342 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10916 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP | Download protocol |
| IHC protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP | Download protocol |
| IP protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP | Download protocol |
| WB protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP | Download protocol |
| mouse | IF Stem Cell Reports Non-genetic Purification of Ventricular Cardiomyocytes from Differentiating Embryonic Stem Cells through Molecular Beacons Targeting IRX-4. Authors - Kiwon Ban View Article |
| human | WB Int J Mol Med Overexpression of CDCA8 promotes the malignant progression of cutaneous melanoma and leads to poor prognosis. Authors - Chao Ci View Article |
| Citations | 5 |
| Dilutions | WB : 1:2000-1:20000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes. Protocols Product Specific Protocols FC protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP Download protocol IHC protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP Download protocol IP protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP Download protocol WB protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Sexual dimorphism of lung immune-regulatory units imprint biased pulmonary fibrosis
Journal: Cell Mol Immunol | Authors: Peng Xiang | Species: mouse | Application: WB - Non-genetic Purification of Ventricular Cardiomyocytes from Differentiating Embryonic Stem Cells through Molecular Beacons Targeting IRX-4.
Journal: Stem Cell Reports | Authors: Kiwon Ban | Species: mouse | Application: IF - SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury.
Journal: iScience | Authors: Anteneh Getachew | Species: human | Application: WB - Overexpression of CDCA8 promotes the malignant progression of cutaneous melanoma and leads to poor prognosis.
Journal: Int J Mol Med | Authors: Chao Ci | Species: human | Application: WB - PRDM16 modulates aspects of cell cycle dynamics and maturation in human iPSC-derived cardiomyocytes.
Journal: Stem Cell Reports | Authors: Kanae Tani
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