MYL6B Polyclonal antibody 16963-1-AP proteintech
$149.00
In stock
SKU
16963-1-AP
| Catalog Number | 16963-1-AP |
| Synonyms | MLC1sa, Myosin light chain 1 slow-twitch muscle A isoform, Myosin light chain 6B |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HuH-7 cells, mouse testis tissue |
| Tested Applications — Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse skeletal muscle tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:3000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Positive WB detected in | HuH-7 cells, mouse testis tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag10683 Product name: Recombinant human MYL6B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-208 aa of BC012425 Sequence: MPPKKDVPVKKPAGPSISKPAAKPAAAGAPPAKTKAEPAVPQAPQKTQEPPVDLSKVVIEFNKDQLEEFKEAFELFDRVGDGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDELKSRRVDFETFLPMLQAVAKNRGQGTYEDYLEGFRVFDKEGNGKVMGAELRHVLTTLGEKMTEEEVETVLAGHEDSNGCINYEAFLKHILSV Predict reactive species |
| Full Name | myosin, light chain 6B, alkali, smooth muscle and non-muscle |
| Calculated Molecular Weight | 208 aa, 23 kDa |
| Observed Molecular Weight | 22-25 kDa |
| GenBank Accession Number | BC012425 |
| Gene Symbol | MYL6B |
| Gene ID (NCBI) | 140465 |
| RRID | AB_3085512 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14649 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for MYL6B antibody 16963-1-AP | Download protocol |
| IHC protocol for MYL6B antibody 16963-1-AP | Download protocol |
| WB protocol for MYL6B antibody 16963-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:500-1:3000 IHC : 1:50-1:500 IF-P : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
MYL6B, also known as MLC1SA, is primarily found in a hexamer consisting of four light chains and two heavy chains. MYL6B is an essential light chain for non-muscle Myosin II (NMII) that is involved in the control of cell adhesion, cell migration and tissue architecture, cargo transport, and endocytosis (PMID: 29439719, 33817240). Protocols Product Specific Protocols IF protocol for MYL6B antibody 16963-1-AP Download protocol IHC protocol for MYL6B antibody 16963-1-AP Download protocol WB protocol for MYL6B antibody 16963-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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