Mono/Di-Methyl-Histone H3 (Lys9) Recombinant monoclonal antibody 80219-1-RR proteintech
$149.00
In stock
SKU
80219-1-RR
| Catalog Number | 80219-1-RR |
| Synonyms | dmk9, H3, H3 Me Lys9, H3 MeK9, H3 Methyl K9 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, ELISA, ChIP-qPCR |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | THP-1 cells, K-562 cells, Jurkat cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, C2C12 cells, HSC-T6 cells, PC-12 cells |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Tested Applications — Positive ChIP-qPCR detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:5000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | THP-1 cells, K-562 cells, Jurkat cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, C2C12 cells, HSC-T6 cells, PC-12 cells |
| Positive IF/ICC detected in | HeLa cells |
| Positive ChIP-qPCR detected in | HeLa cells |
| Western Blot (WB) | WB : 1:2000-1:5000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| Tested Reactivity | human, mouse, rat |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | histone cluster 1, H3a |
| Observed Molecular Weight | 15-18 kDa |
| GenBank Accession Number | BC066245 |
| Gene Symbol | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| RRID | AB_2918878 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P68431 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for Mono/Di-Methyl-Histone H3 (Lys9) antibody 80219-1-RR | Download protocol |
| WB protocol for Mono/Di-Methyl-Histone H3 (Lys9) antibody 80219-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:2000-1:5000 IF/ICC : 1:200-1:800 CHIP-QPCR : 1:10-1:100 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 1E12 |
| Conjugation | Unconjugated |
Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified. Methylation of Histone H3 at lysine 9 is linked to transcriptional repression. This antibody is specific to monomethyl or dimethyl-Histone H3 while it does not recognize trimethyl-Histone H3 (Lys9). It is also named as H3K9me1/2. Protocols Product Specific Protocols IF protocol for Mono/Di-Methyl-Histone H3 (Lys9) antibody 80219-1-RR Download protocol WB protocol for Mono/Di-Methyl-Histone H3 (Lys9) antibody 80219-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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