CoraLite® Plus 488-conjugated 6*His, His-Tag Monoclonal antibody CL488-66005 proteintech
$189.00
In stock
SKU
CL488-66005
| Catalog Number | CL488-66005 |
| Synonyms | 6*His, His Tag, His Tag, 6*His, 6*his tag, 6*his,His tag |
| Host | Mouse |
| Reactivity | recombinant protein and More (3) |
| Reactivity | recombinant protein, human, mouse, chicken |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | IF/ICC, FC (Intra) |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive IF/ICC detected in | Transfected HEK-293 cells |
| Tested Applications — Positive FC (Intra) detected in | Transfected HEK-293 cells |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, chicken |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | 6*His, His-Tag |
| Calculated Molecular Weight | 0.84 kDa |
| RRID | AB_2883223 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Purification Method | Protein G purification |
| UNIPROT ID | HISTAG |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| FC protocol for CL Plus 488 6*His, His-Tag antibody CL488-66005 | Download protocol |
| IF protocol for CL Plus 488 6*His, His-Tag antibody CL488-66005 | Download protocol |
| Citations | 21 |
| Dilutions | IF/ICC : 1:50-1:500 FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1B7G5 |
| Conjugation | CoraLite® Plus 488 |
Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein. His-tag is often used for affinity purification and binding assays. Expressed. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells. A His-tag (polyhistidine tag) consists of at least six histidine residues that are located at the N- or C-terminus of recombinant proteins. It is commonly used for affinity purification and protein binding experiments. The recombinant protein has only 6 histidine residues as a tag. Is this sufficient to be detected by your antibody? Is the His-tag antibody able to detect N-terminal and C-terminal His tags? The His-tag antibody was raised using a 6-His tag peptide as the immunogen, which is ~1 kDa. Some scientists use more than 6 histidine residues as a tag for recombinant proteins to increase affinity to metal ions. A 6-histidine tag is sufficient for specific recognition by the His-tag antibody. It is able to recognize both N-terminal and C-terminal His tags of recombinant proteins produced in various expression systems, including bacteria, yeasts, insect, and mammalian cells. After purification, I can detect my protein by western blotting using the His-tag antibody but can also see additional bands running lower. What might they represent? They may represent cleavage products of the recombinant protein. Unspecific protein cleavage can occur during protein production (e.g., in bacteria) or protein purification post the cell lysis steps. Changing expression conditions (temperature, time, induction methods, medium) and using protease inhibitors during purification helps to minimize protein degradation. Additional steps, such as ion exchange, size exclusion, or chromatography, allow isolation of the full-length product. Can a His-tag antibody be used in applications other than western blotting? Yes, this antibody has been successfully used for immunofluorescence (IF), immunoprecipitation (IP), and indirect ELISA analysis. Protocols Product Specific Protocols FC protocol for CL Plus 488 6*His, His-Tag antibody CL488-66005 Download protocol IF protocol for CL Plus 488 6*His, His-Tag antibody CL488-66005 Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Breakthrough infection elicits hypermutated IGHV3-53/3-66 public antibodies with broad and potent neutralizing activity against SARS-CoV-2 variants including the emerging EG.5 lineages
Journal: PLoS Pathog | Authors: Ling Li - Apostichopus japonicus matrix metalloproteinase-16 might act as a pattern recognition receptor.
Journal: Fish Shellfish Immunol | Authors: Jingwei Jiang - Human adenovirus protein VII inhibits type I IFN production by antagonizing viral RNA sensor RIG-I.
Journal: Cell Rep | Authors: Pei-Hong Yu - Characterization of a novel short-type peptidoglycan recognition protein from the sea cucumber Apostichopus japonicus
Journal: Int J Biol Macromol | Authors: Pingzhe Jiang | Application: IF - Conserved deletion of two consecutive glycine residues in the 100K protein is a critical determinant of FAdV-11 pathogenicity.
Journal: Virulence | Authors: Baiyu Wang | Application: IF - A novel shark VNAR antibody-based immunotoxin targeting TROP-2 for cancer therapy
Journal: Acta Pharm Sin B | Authors: Xiaozhi Xi
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