Speedy™ Mouse LIX/CXCL5 One-Step ELISA Kit SE60078 proteintech
$449.00
In stock
SKU
SE60078
| Product name | Speedy™ Mouse LIX/CXCL5 One-Step ELISA Kit |
| Synonyms | C-X-C motif chemokine 5, Cxcl5, Cytokine LIX, GCP-2(1-78), GCP-2(9-78) |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernate, Urine, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.5 pg/mL |
| Range | 3.13-200 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20311 |
| Sample Type | Average Range |
| Mouse serum | 101% 87%-113% |
| Cell culture supernate | 101% 96%-110% |
| Urine | 96% 84%-117% |
| Tissue lysate | 107% 76%-120% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 95.7 4.0 4.2 |
| 2 | 16 26.2 0.9 3.4 |
| 3 | 16 14.4 0.4 2.8 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
LPS-induced CXC chemokine (LIX) is a murine chemokine similar to two human chemokines, ENA-78 (CXCL5) and GCP-2 (CXCL6). Mouse LIX can be produced by many immune cells, such as macrophages, eosinophils , as well as non-immune cells including mesothelial cells, and fibroblasts. Increased serum LIX has been observed in obesity and insulin resistance in mice and humans and is associated with complications of diabetes including nephropathy and atherosclerosis. It is mainly produced when induced by LPS, IL-17 and/or TNF-α. According to the previous studies, cancer cell autonomous secretion of LIX as sufficient to drive infiltration of mature, protumorigenic neutrophils in a mouse model of non-small cell lung cancer (NSCLC). Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms C-X-C motif chemokine 5, Cxcl5, Cytokine LIX, GCP-2(1-78), GCP-2(9-78) FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
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