ChromoTek Myc-Trap® Magnetic Agarose, Kit for Immunoprecipitation ytmak proteintech
$625.00
In stock
SKU
ytmak
| Synonyms | Myc tag, Myc, Myc-trap, EQKLISEEDL |
| Host | Alpaca |
| Formulation | United States |
| Applications | IP, CoIP, ChIP, RIP |
| Host / Isotype | IP, CoIP, ChIP, RIP |
| Specificity/Target | Myc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound. |
| Binding capacity | 17.5 μg of recombinant Myc-tagged protein (~42 kDa) per 25 μL bead slurry |
| Conjugate | Magnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads) |
| Elution buffer | For acidic elution |
| Wash buffer compatibility | 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100 |
| Type | Nanobody |
| Class | Recombinant |
| Affinity (K D ) | 1x Myc-tag: Dissociation constant K D of 500 nM 2x Myc-tag: Dissociation constant K D of 0.5 nM |
| Compatibility with mass spectrometry | The Myc-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2631370 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
| Component | Description |
| Myc-Trap® Magnetic Agarose | 20 reactions (500 µL) |
| Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| IP,CoIP | Cell Death Dis Transcription-dependent and -independent functions of Drosophila p53 isoforms in the induction of apoptosis and senescence-associated tumorigenesis Authors - Marina Pérez-Aguilera View Article |
| CoIP | Curr Biol Atg8/LC3 controls systemic nutrient surplus signaling in flies and humans Authors - Aditi Madan View Article |
| IP | J Cell Sci Aggregation-prone Tau impairs mitochondrial import, which affects organelle morphology and neuronal complexity Authors - Hope I Needs View Article |
| FH Mona (Verified Customer) (08-27-2022) | I could pull down a Myc tagged protein with this kit. The efficiency was 100%. Highly recommended. Applications: Immunoprecipitation Cell Tissue Type: HeLa cell |
Description
The Myc-Trap Magnetic Agarose Kit contains Myc-Trap Magnetic Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of Myc-tagged proteins and their interacting factors. • Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains on the SDS-PAGE • Stable under harsh washing conditions • Suitable for downstream mass spec analysis
Publications
- Transcription-dependent and -independent functions of Drosophila p53 isoforms in the induction of apoptosis and senescence-associated tumorigenesis
Journal: Cell Death Dis | Authors: Marina Pérez-Aguilera | Species: IP,CoIP | Application: Cell Death Dis Transcription-dependent and -independent functions of Drosophila p53 isoforms in the induction of apoptosis and senescence-associated tumorigenesis Authors - Marina Pérez-Aguilera View Article - Atg8/LC3 controls systemic nutrient surplus signaling in flies and humans
Journal: Curr Biol | Authors: Aditi Madan | Species: CoIP | Application: Curr Biol Atg8/LC3 controls systemic nutrient surplus signaling in flies and humans Authors - Aditi Madan View Article - Aggregation-prone Tau impairs mitochondrial import, which affects organelle morphology and neuronal complexity
Journal: J Cell Sci | Authors: Hope I Needs | Species: IP | Application: J Cell Sci Aggregation-prone Tau impairs mitochondrial import, which affects organelle morphology and neuronal complexity Authors - Hope I Needs View Article - A regulatory module integrates lipid metabolism and cell wall remodeling to establish the seed coat palisade barrier.
Journal: Plant Cell | Authors: Zhichao Lu | Application: Plant Cell A regulatory module integrates lipid metabolism and cell wall remodeling to establish the seed coat palisade barrier. Authors - Zhichao Lu View Article
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