NACA Polyclonal antibody 32235-1-AP proteintech
$149.00
In stock
SKU
32235-1-AP
| Catalog Number | 32235-1-AP |
| Synonyms | Alpha NAC, Hom s 2, HSD48, NAC alpha, NACA1 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, U-87 MG cells, mouse brain tissue, rat brain tissue |
| Tested Applications — Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:6000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | HeLa cells, U-87 MG cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag37704 Product name: Recombinant human NACA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-215 aa of BC106041 Sequence: MPGEATETVPATEQELPQPQAETGSGTESDSDESVPELEEQDSTQATTQQAQLAAAAEIDEEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELTM Predict reactive species |
| Full Name | nascent polypeptide-associated complex alpha subunit |
| Calculated Molecular Weight | 215 aa, 23 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC106041 |
| Gene Symbol | NACA |
| Gene ID (NCBI) | 4666 |
| RRID | AB_3670227 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q13765 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for NACA antibody 32235-1-AP | Download protocol |
| WB protocol for NACA antibody 32235-1-AP | Download protocol |
| Citations | 1 |
| Dilutions | WB : 1:1000-1:6000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
NACA (NAC-alpha), or Nascent polypeptide-associated complex subunit alpha, is a component of the nascent polypeptide-associated complex (NAC). NACA is a key subunit of the NAC complex that assists in the proper folding of newly synthesized proteins and prevents the formation of harmful protein aggregates, playing a vital role in cellular protein quality control and stress response mechanisms. The calculated MW of NACA is 23 kDa, and the observed MW is 35 kDa, which is due to phosphorylation modification (PMID: 30948508, 36107769). Protocols Product Specific Protocols IHC protocol for NACA antibody 32235-1-AP Download protocol WB protocol for NACA antibody 32235-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Structural basis of cotranslational protein N-terminal acetylation by NatB in human cells.
Journal: Nat Commun | Authors: Natalia Silva Alves
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