Napsin A Polyclonal antibody 16558-1-AP proteintech
$149.00
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SKU
16558-1-AP
| Catalog Number | 16558-1-AP |
| Synonyms | NAPSA, Asp 4, ASP4, Aspartyl protease 4, EC:3.4.23.- |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse lung tissue |
| Tested Applications — Positive IHC detected in | human lung cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HUVEC cells |
| Tested Applications — Positive FC (Intra) detected in | A549 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 4.00 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | mouse lung tissue |
| Positive IHC detected in | human lung cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HUVEC cells |
| Positive FC (Intra) detected in | A549 cells |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 4.00 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag9786 Product name: Recombinant human NAPSA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-420 aa of BC017842 Sequence: MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRTLNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG Predict reactive species |
| Full Name | napsin A aspartic peptidase |
| Calculated Molecular Weight | 420 aa, 45 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC017842 |
| Gene Symbol | Napsin A |
| Gene ID (NCBI) | 9476 |
| RRID | AB_2878278 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O96009 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for Napsin A antibody 16558-1-AP | Download protocol |
| IF protocol for Napsin A antibody 16558-1-AP | Download protocol |
| IHC protocol for Napsin A antibody 16558-1-AP | Download protocol |
| WB protocol for Napsin A antibody 16558-1-AP | Download protocol |
| human | IHC Cell Biol Toxicol Harnessing machine learning-driven multiomics integration: deciphering programmed cell death networks for prognostication and immunotherapy prediction in lung adenocarcinoma. Authors - Wuguang Chang View Article |
| Citations | 1 |
| Dilutions | WB : 1:500-1:1000 IHC : 1:50-1:500 IF/ICC : 1:50-1:500 FC (INTRA) : 4.00 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963). Protocols Product Specific Protocols FC protocol for Napsin A antibody 16558-1-AP Download protocol IF protocol for Napsin A antibody 16558-1-AP Download protocol IHC protocol for Napsin A antibody 16558-1-AP Download protocol WB protocol for Napsin A antibody 16558-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Harnessing machine learning-driven multiomics integration: deciphering programmed cell death networks for prognostication and immunotherapy prediction in lung adenocarcinoma.
Journal: Cell Biol Toxicol | Authors: Wuguang Chang | Species: human | Application: IHC
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