NARS Monoclonal antibody 67711-1-Ig proteintech
$149.00
In stock
SKU
67711-1-Ig
| Catalog Number | 67711-1-Ig |
| Synonyms | ASNRS, Asparagine tRNA ligase, asparaginyl tRNA synthetase, NARS, NARS1 |
| Host | Mouse |
| Reactivity | Human, mouse, rat |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Mouse / IgG2a |
| Tested Applications — Positive WB detected in | LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells |
| Tested Applications — Positive IHC detected in | human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Positive WB detected in | LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells |
| Positive IHC detected in | human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Tested Reactivity | Human, mouse, rat |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag6656 Product name: Recombinant human NARS protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 199-548 aa of BC001687 Sequence: ELSCDFWELIGLAPAGGADNLINEESDVDVQLNNRHMMIRGENMSKILKARSMVTRCFRDHFFDRGYYEVTPPTLVQTQVEGGATLFKLDYFGEEAFLTQSSQLYLETCLPALGDVFCIAQSYRAEQSRTRRHLAEYTHVEAECPFLTFDDLLNRLEDLVCDVVDRILKSPAGSIVHELNPNFQPPKRPFKRMNYSDAIVWLKEHDVKKEDGTFYEFGEDIPEAPERLMTDTINEPILLCRFPVEIKSFYMQRCPEDSRLTESVDVLMPNVGEIVGGSMRIFDSEEILAGYKREGIDPTPYYWYTDQRKYGTCPHGGYGLGLERFLTWILNRYHIRDVCLYPRFVQRCTP Predict reactive species |
| Full Name | asparaginyl-tRNA synthetase |
| Calculated Molecular Weight | 63 kDa |
| Observed Molecular Weight | 63 kDa |
| GenBank Accession Number | BC001687 |
| Gene Symbol | NARS |
| Gene ID (NCBI) | 4677 |
| RRID | AB_2882901 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | O43776 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for NARS antibody 67711-1-Ig | Download protocol |
| IHC protocol for NARS antibody 67711-1-Ig | Download protocol |
| WB protocol for NARS antibody 67711-1-Ig | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:2000-1:8000 IF/ICC : 1:400-1:1600 |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Clone Number | 4G5G12 |
| Conjugation | Unconjugated |
Asparagine-tRNA ligase (NARS) belongs to the class-II aminoacyl-tRNA synthetase family. Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn) (PubMed: 9421509). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells. The NARS levels were higher in tumor tissues compared with adjacent normal tissues, and positively associated with LN metastasis. NARS may serve as a potential biomarker for lung adenocarcinoma (ADC) diagnosis/prognosis (PMID: 27161446). Protocols Product Specific Protocols IF protocol for NARS antibody 67711-1-Ig Download protocol IHC protocol for NARS antibody 67711-1-Ig Download protocol WB protocol for NARS antibody 67711-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
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