IkB Alpha Polyclonal antibody 18220-1-AP proteintech
$149.00
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SKU
18220-1-AP
| Catalog Number | 18220-1-AP |
| Synonyms | IKBA, IκB α, NFKBIA, Ikβ-α, IkB-alpha |
| Host | Rabbit |
| Reactivity | human and More (2) |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HL-60 cells, HeLa cells, Jurkat cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | human kidney tissue, human liver tissue, human prostate cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:3000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HL-60 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue, human liver tissue, human prostate cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag12979 Product name: Recombinant human NFKBIA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 20-217 aa of BC004983 Sequence: LKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNG Predict reactive species |
| Full Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC004983 |
| Gene Symbol | IkB Alpha |
| Gene ID (NCBI) | 4792 |
| RRID | AB_2298441 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P25963 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for IkB Alpha antibody 18220-1-AP | Download protocol |
| IHC protocol for IkB Alpha antibody 18220-1-AP | Download protocol |
| IP protocol for IkB Alpha antibody 18220-1-AP | Download protocol |
| WB protocol for IkB Alpha antibody 18220-1-AP | Download protocol |
| human | WB Cancer Biol Ther MiR-376a-3p increases cell apoptosis in acute myeloid leukemia by targeting MT1X. Authors - Xiangke Xin View Article |
| rat | WB J Neuroinflammation Upregulation of neuronal zinc finger protein A20 expression is required for electroacupuncture to attenuate the cerebral inflammatory injury mediated by the nuclear factor-kB signaling pathway in cerebral ischemia/reperfusion rats. Authors - Jian Zhan View Article |
| mouse | WB Biomed Pharmacother Tubeimoside I improves survival of mice in sepsis by inhibiting inducible nitric oxide synthase expression. Authors - Minghao Luo View Article |
| mouse,rat | WB Eur J Pharmacol Tubeimoside I protects against sepsis-induced cardiac dysfunction via SIRT3. Authors - Zhe Cheng View Article |
| Citations | 19 |
| Dilutions | WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime. Protocols Product Specific Protocols IF protocol for IkB Alpha antibody 18220-1-AP Download protocol IHC protocol for IkB Alpha antibody 18220-1-AP Download protocol IP protocol for IkB Alpha antibody 18220-1-AP Download protocol WB protocol for IkB Alpha antibody 18220-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Activation of the NF-¿B pathway as a mechanism of alcohol enhanced progression and metastasis of human hepatocellular carcinoma.
Journal: Mol Cancer | Authors: Fei Wang | Species: human | Application: WB - Electroacupuncture Suppresses the NF-κB Signaling Pathway by Upregulating Cylindromatosis to Alleviate Inflammatory Injury in Cerebral Ischemia/Reperfusion Rats.
Journal: Front Mol Neurosci | Authors: Jin Jiang | Species: rat | Application: WB - Upregulation of neuronal zinc finger protein A20 expression is required for electroacupuncture to attenuate the cerebral inflammatory injury mediated by the nuclear factor-kB signaling pathway in cerebral ischemia/reperfusion rats.
Journal: J Neuroinflammation | Authors: Jian Zhan | Species: rat | Application: WB - Tubeimoside I improves survival of mice in sepsis by inhibiting inducible nitric oxide synthase expression.
Journal: Biomed Pharmacother | Authors: Minghao Luo | Species: mouse | Application: WB - MiR-376a-3p increases cell apoptosis in acute myeloid leukemia by targeting MT1X.
Journal: Cancer Biol Ther | Authors: Xiangke Xin | Species: human | Application: WB - Tubeimoside I protects against sepsis-induced cardiac dysfunction via SIRT3.
Journal: Eur J Pharmacol | Authors: Zhe Cheng | Species: mouse,rat | Application: WB - MiR-376a-3p increases cell apoptosis in acute myeloid leukemia by targeting MT1X.
Journal: Cancer Biol Ther | Authors: Xiangke Xin | Species: human | Application: WB - The Effect of Bergenin on Isonicotinic Acid Hydrazide and Rifampicin-Induced Liver Injury Revealed by RNA Sequencing
Journal: Molecules | Authors: Ting Li | Species: mouse | Application: WB - Peroxiredoxin 6 attenuates ischemia‑ and hypoxia‑induced liver damage of brain‑dead donors.
Journal: Mol Med Rep | Authors: Qiang Tu | Species: human | Application: WB - Quercetin attenuates high glucose-induced VEGFA expression in ARPE-19 cells by inhibiting ROS generation, p38 MAPK phosphorylation, and NF-κB activation.
Journal: Sci Rep | Authors: Pei-Kang Liu | Species: human | Application: WB - Yixinyuan Gaofang ameliorates myocardial infarction in rats by inhibiting the TNF/NF-κB pathway.
Journal: Sci Rep | Authors: Lan Xiao | Species: rat | Application: WB - Electroacupuncture Suppresses the NF-κB Signaling Pathway by Upregulating Cylindromatosis to Alleviate Inflammatory Injury in Cerebral Ischemia/Reperfusion Rats.
Journal: Front Mol Neurosci | Authors: Jin Jiang | Species: rat | Application: WB - Aerobic exercise ameliorates atherosclerosis-induced cognitive impairment via hippocampal IL-33/NF-κB signaling modulation
Journal: Front Physiol | Authors: Jianwei Peng - RAD51 promotes osteogenic differentiation and inhibits DNA damage in osteoporosis though regulating cGAS-STING signaling pathway.
Journal: Regen Ther | Authors: Minli Qiu | Species: mouse | Application: WB - Xanthoxylin Regulating the Inflammatory Microenvironment After Spinal Cord Injury Through Inhibition of the NF-κB Signaling Pathway.
Journal: Neuromolecular Med | Authors: Zihao Fan | Species: mouse | Application: WB - Tubeimoside I improves survival of mice in sepsis by inhibiting inducible nitric oxide synthase expression.
Journal: Biomed Pharmacother | Authors: Minghao Luo | Species: mouse | Application: WB - 18β-glycyrrhetinic acid-amantadine hybrid: Synthesis and anti-EMCV activity via NF-κB modulation.
Journal: Bioorg Med Chem | Authors: Weijiao Xue | Species: human | Application: WB - A20-Binding Inhibitor of NF-κB 1 Ameliorates Neuroinflammation and Mediates Antineuroinflammatory Effect of Electroacupuncture in Cerebral Ischemia/Reperfusion Rats.
Journal: Evid Based Complement Alternat Med | Authors: Xueling Zhou | Species: rat | Application: WB - Magnoflorine ameliorates cartilage degradation in osteoarthritis through inhibition of mitochondrial reactive oxygen species-mediated activation of the NLRP3 inflammasome
Journal: J Asian Nat Prod Res | Authors: Yi Peng | Species: human | Application: WB
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