Lipocalin-2/NGAL Recombinant monoclonal antibody 82841-1-RR proteintech
$149.00
In stock
SKU
82841-1-RR
| Catalog Number | 82841-1-RR |
| Synonyms | LCN2, lipocalin 2, NGAL, 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | human saliva, HT-29 cells, SW480 cells |
| Tested Applications — Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | human saliva, HT-29 cells, SW480 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Eg0268 Product name: Recombinant Human NGAL/Lipocalin-2 protein (His Tag) Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-10*HIS Domain: 21-198 aa of NP_005555.2 Sequence: QDSTSDLIPAPPLSKVPLQQNFQDNQFQGKWYVVGLAGNAILREDKDPQKMYATIYELKEDKSYNVTSVLFRKKKCDYWIRTFVPGCQPGEFTLGNIKSYPGLTSYLVRVVSTNYNQHAMVFFKKVSQNREYFKITLYGRTKELTSELKENFIRFSKSLGLPENHIVFPVPIDQCIDG Predict reactive species |
| Full Name | lipocalin 2 |
| Calculated Molecular Weight | 23kd |
| Observed Molecular Weight | 22 kDa |
| GenBank Accession Number | NP_005555.2 |
| Gene Symbol | NGAL/LCN2 |
| Gene ID (NCBI) | 3934 |
| RRID | AB_3086550 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P80188 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for Lipocalin-2/NGAL antibody 82841-1-RR | Download protocol |
| WB protocol for Lipocalin-2/NGAL antibody 82841-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:2000-1:10000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 2G1 |
| Conjugation | Unconjugated |
Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264). Protocols Product Specific Protocols IHC protocol for Lipocalin-2/NGAL antibody 82841-1-RR Download protocol WB protocol for Lipocalin-2/NGAL antibody 82841-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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