NINJ2 Monoclonal antibody 66202-1-Ig proteintech
$149.00
In stock
SKU
66202-1-Ig
| Catalog Number | 66202-1-Ig |
| Synonyms | 5C5B2, Nerve injury-induced protein 2, Ninjurin-2 |
| Host | Mouse |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Mouse / IgG2b |
| Tested Applications — Positive WB detected in | HeLa cells, SiHa cells, MOLT-4 cells, HuT 78 cells, Karpas-299 cells |
| Tested Applications — Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | human tonsillitis tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Positive WB detected in | HeLa cells, SiHa cells, MOLT-4 cells, HuT 78 cells, Karpas-299 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Tested Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag5343 Product name: Recombinant human NINJ2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 17-158 aa of BC057766 Sequence: MESARENIDLQPGSSDPRSQPINLNHYATKKSVAESMLDVALFMSNAMRLKAVLEQGPSSHYYTTLVTLISLSLLLQVVIGVLLVVIARLNLNEVEKQWRLNQLNNAATILVFFTVVINVFITAFGAHKTGFLAARASRNPL Predict reactive species |
| Full Name | ninjurin 2 |
| Calculated Molecular Weight | 142 aa, 16 kDa |
| GenBank Accession Number | BC057766 |
| Gene Symbol | NINJ2 |
| Gene ID (NCBI) | 4815 |
| RRID | AB_2881593 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NZG7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for NINJ2 antibody 66202-1-Ig | Download protocol |
| IHC protocol for NINJ2 antibody 66202-1-Ig | Download protocol |
| WB protocol for NINJ2 antibody 66202-1-Ig | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:20-1:200 IF-P : 1:200-1:800 |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Clone Number | 5C5B2 |
| Conjugation | Unconjugated |
NINJ2 (Ninjurin2) is a member of the ninjurin family of adhesion molecules which mediate cell-to-cell and cell-to-extracellular matrix interactions during development, differentiation, and regeneration of the peripheral nervous system. The gene encodes NINJ2 is located on chromosome 12p13. NINJ2 mRNA is widely expressed in adult human tissues, with highest level in bone marrow, followed by peripheral leukocytes, lung, and lymph nodes. In the peripheral nervous system, NINJ2 is expressed constitutively in mature sensory and enteric neurons. The expression of NINJ2 is upregulated after nerve injury in Schwann cells, suggesting that it may promote nerve regeneration. It may also play an important role in the pathogenesis of inflammatory disorder. (PMID: 10627596) Protocols Product Specific Protocols IF protocol for NINJ2 antibody 66202-1-Ig Download protocol IHC protocol for NINJ2 antibody 66202-1-Ig Download protocol WB protocol for NINJ2 antibody 66202-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
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