NMT1 Monoclonal antibody 67984-1-Ig proteintech
$149.00
In stock
SKU
67984-1-Ig
| Catalog Number | 67984-1-Ig |
| Synonyms | 2D9B9, EC:2.3.1.-, EC:2.3.1.97, Glycylpeptide N-tetradecanoyltransferase 1, HsNMT1 |
| Host | Mouse |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | LNCaP cells, HeLa cells, L02 cells, PC-3 cells, SKOV-3 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Tested Applications — Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Positive WB detected in | LNCaP cells, HeLa cells, L02 cells, PC-3 cells, SKOV-3 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag20541 Product name: Recombinant human NMT1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-301 aa of BC006569 Sequence: MADESETAVKPPAPPLPQMMEGNGNGHEHCSDCENEEDNSYNRGGLSPANDTGAKKKKKKQKKKKEKGSETDSAQDQPVKMNSLPAERIQEIQKAIELFSVGQGPAKTMEEASKRSYQFWDTQPVPKLGEVVNTHGPVEPDKDNIRQEPYTLPQGFTWDALDLGDRGVLKELYTLLNENYVEDDDNMFRFDYSPEFLLWALRPPGWLPQWHCGVRVVSSRKLVGFISAIPANIHIYDTEKKMVEINFLCVHKKLRSKRVAPVLIREITRRVHLEGIFQAVYTAGVVLPKPVGTCRYWHRSL Predict reactive species |
| Full Name | N-myristoyltransferase 1 |
| Calculated Molecular Weight | 496 aa, 57 kDa |
| Observed Molecular Weight | 50-60 kDa |
| GenBank Accession Number | BC006569 |
| Gene Symbol | NMT1 |
| Gene ID (NCBI) | 4836 |
| RRID | AB_2918733 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | P30419 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for NMT1 antibody 67984-1-Ig | Download protocol |
| IHC protocol for NMT1 antibody 67984-1-Ig | Download protocol |
| WB protocol for NMT1 antibody 67984-1-Ig | Download protocol |
| human | WB J Cell Sci HIV-1 N-myristoylation-dependent hijacking of late endosomes/lysosomes to drive Gag assembly in macrophages Authors - Gabriel I Guajardo-Contreras View Article |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:500-1:2000 IF/ICC : 1:400-1:1600 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 2D9B9 |
| Conjugation | Unconjugated |
NMT1 is a N-myristoyltransferase responsible for the transfer of myristate from CoA to an amino-terminal glycine of many eukaryotic proteins, which facilitates the targeting of proteins to membrane surfaces and is essential for viability of the organism. Insertional mutagenesis of the Nmt1 gene in Saccharomyces cerevisiae causes recessive lethality. Humans and mice possess two distinct but structurally similar enzymes, NMT1 and NMT2, ubiquitously expressed in most human and mouse tissues. Western analysis revealed that there are 4 isoforms of NMT1 with apparent molecular masses ranging from 49 to 68 kDa. In cell fractionation studies, the 68-kDa NMT1 isoform and NMT2 were present in both membrane and cytoplasmic fractions, while the smaller NMT1 isoforms were predominantly cytoplasmic. Protocols Product Specific Protocols IF protocol for NMT1 antibody 67984-1-Ig Download protocol IHC protocol for NMT1 antibody 67984-1-Ig Download protocol WB protocol for NMT1 antibody 67984-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- HIV-1 N-myristoylation-dependent hijacking of late endosomes/lysosomes to drive Gag assembly in macrophages
Journal: J Cell Sci | Authors: Gabriel I Guajardo-Contreras | Species: human | Application: WB
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