NSUN4 Polyclonal antibody 29786-1-AP proteintech
$149.00
In stock
SKU
29786-1-AP
| Catalog Number | 29786-1-AP |
| Synonyms | RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, RIP, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, K-562 cells, SH-SY5Y cells |
| Tested Applications — Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:6000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | HepG2 cells, K-562 cells, SH-SY5Y cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag31821 Product name: Recombinant human NSUN4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 26-284 aa of BC014441 Sequence: RYKKKWAATEPKFPAVRLALQNFDMAYSVQFGDLWPSIRVSLLSEQKYGALVNNFAAWDHVSAKLEQLSAKDFVNEAISHWELQSEGGQSAAPSPASWACSPNLRCFTFDRGDISRFPPARPGSLGVMEYYLMDAASLLPVLALGLQPGDIVLDLCAAPGGKTLALLQTGCCRNLAANDLSPSRIARLQKILHSYVPEEIRDGNQVRVTSWDGRKWGELEGDTYDRVLVDVPCTTDRHSLHEEENNIFKRSRKKERQIL Predict reactive species |
| Full Name | NOL1/NOP2/Sun domain family, member 4 |
| Calculated Molecular Weight | 384 aa, 43 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC014441 |
| Gene Symbol | NSUN4 |
| Gene ID (NCBI) | 387338 |
| RRID | AB_2935480 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96CB9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for NSUN4 antibody 29786-1-AP | Download protocol |
| WB protocol for NSUN4 antibody 29786-1-AP | Download protocol |
| human | WB,RIP,IHC Cancers (Basel) NSUN4 Suppresses Ferroptosis Through m(5)C-Dependent Stabilization of C-MYC and Activation of the PI3K/Akt Signaling Pathway in Cervical Cancer. Authors - Duancheng Tian KD Validated View Article |
| Citations | 3 |
| Dilutions | WB : 1:1000-1:6000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
NSUN4 belongs to a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases). MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts with MTERF4 to facilitate mitoribosomal assembly. The calculated MW of NSUN4 is 43 kDa, 29786-1-AP can detect the band around 40 kDa.(PMID:22949673, 24516400) Protocols Product Specific Protocols IHC protocol for NSUN4 antibody 29786-1-AP Download protocol WB protocol for NSUN4 antibody 29786-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- NSUN4 Facilitates the Activity of Oncogenic Protein CDC20 to Promote NSCLC Development by Mediating m5C Modification of CDC20 mRNA
Journal: Thorac Cancer | Authors: Zhilong Li | Species: human | Application: WB - NSUN4 Suppresses Ferroptosis Through m(5)C-Dependent Stabilization of C-MYC and Activation of the PI3K/Akt Signaling Pathway in Cervical Cancer.
Journal: Cancers (Basel) | Authors: Duancheng Tian KD Validated | Species: human | Application: WB,RIP,IHC - NSUN2-mediated m5C modification of BHLF1 RNA facilitates the lytic replication of Epstein-Barr virus.
Journal: Cell Rep | Authors: Tian-Liang Xia
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