NSUN7 Polyclonal antibody 17546-1-AP proteintech
$149.00
In stock
SKU
17546-1-AP
| Catalog Number | 17546-1-AP |
| Synonyms | FLJ14001 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, RIP, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse liver tissue, mouse testis tissue, rat liver tissue |
| Tested Applications — Positive IHC detected in | rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Positive WB detected in | mouse liver tissue, mouse testis tissue, rat liver tissue |
| Positive IHC detected in | rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag11650 Product name: Recombinant human NSUN7 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 125-475 aa of BC036568 Sequence: SLIIVMLYDFQDRKFQTRVLSDNEEPISEVQEVENLLNSFKIKLAAALARCRIKHDALSIYHILPETVRKQELRASTLPLYAWINTCKISPEEVYNNLKRRGYNKVKSVLHIDDKVFAVDQHCYDVLIFPSHLKNDLINIDLFKDYKLIFQDKSRSLAVHSVKALLNMDDDVLMVNTGSWYTVAHMSILTNNNTSKVFVCGVQSQAKDPDLKTLFTKIGCKNIEILHEKFINIESKDHRLQKVKVILLLPRCSGLGVSNPVEFILNEHEDTEFLKDHSQGGISVDKLHVLAQQQYEQLTHAMKFTKAQAVVYCTCSVFPEENEAVVKKALEFQDLGNKGQPYSGTLLRQCL Predict reactive species |
| Full Name | NOL1/NOP2/Sun domain family, member 7 |
| Calculated Molecular Weight | 718 aa, 81 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC036568 |
| Gene Symbol | NSUN7 |
| Gene ID (NCBI) | 79730 |
| RRID | AB_2878415 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NE18 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for NSUN7 antibody 17546-1-AP | Download protocol |
| WB protocol for NSUN7 antibody 17546-1-AP | Download protocol |
| human | WB J Diabetes Investig NSUN2-mediated m(5)C methylation modification regulates trophoblast cell proliferation, apoptosis, and autophagy in gestational diabetes mellitus. Authors - Jiujiu Liu View Article |
| human,rat | WB,RIP FASEB J NSUN2 Exacerbates Renal Fibrosis by Inducing Epithelial-Mesenchymal Transition Through Mediating m5C Modification of BRD4. Authors - Chongxiang Xiong View Article |
| Citations | 5 |
| Dilutions | WB : 1:500-1:1000 IHC : 1:250-1:1000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
NSUN7 (NOP2/Sun RNA methyltransferase family member 7) is involved in mitochondrial rRNA processing in post-meiotic sperm and is predicted to act upstream of or within flagellated sperm motility and sperm mitochondrion organization. In humans, Nsun7 encodes a protein with 718-amino acid and 3697-bp belonging to the methyltransferase superfamily (PMID: 37173708). The failure to produce NSUN7 would lead to the impairment in activity and motility of sperm flagella, leading to sperm motility deficit and infertility (PMID: 25702163). Protocols Product Specific Protocols IHC protocol for NSUN7 antibody 17546-1-AP Download protocol WB protocol for NSUN7 antibody 17546-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Comprehensive Analysis of 5-Methylcytosine (m5C) Regulators and the Immune Microenvironment in Pancreatic Adenocarcinoma to Aid Immunotherapy.
Journal: Front Oncol | Authors: Ronglin Wang | Species: human | Application: WB - Enoxaparin alleviates preeclampsia by enhancing trophoblast function via the NSUN2-mediated m(5)C methylation of PAX3 mRNA.
Journal: Eur J Med Res | Authors: Xuan Zhong | Species: human | Application: WB - NSUN2-mediated m(5)C methylation modification regulates trophoblast cell proliferation, apoptosis, and autophagy in gestational diabetes mellitus.
Journal: J Diabetes Investig | Authors: Jiujiu Liu | Species: human | Application: WB - NSUN2 Exacerbates Renal Fibrosis by Inducing Epithelial-Mesenchymal Transition Through Mediating m5C Modification of BRD4.
Journal: FASEB J | Authors: Chongxiang Xiong | Species: human,rat | Application: WB,RIP - NSUN2-mediated m5C modification of BHLF1 RNA facilitates the lytic replication of Epstein-Barr virus.
Journal: Cell Rep | Authors: Tian-Liang Xia
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