ChromoTek Nano-CaptureLigand®, anti-human/rabbit IgG (Fc) recombinant VHH, biotinylated shurbGB-1 proteintech
$137.00
In stock
SKU
shurbGB-1
| Synonyms | immunoglobulin, human IgG, Fc domain, Rabbit IgG |
| Host | Alpaca |
| Reactivity | Human IgG1, IgG2, IgG3, and IgG4 Fc-fragments Rabbit IgG Fc-fragment |
| Formulation | United States |
| Applications | BLI, SPR, ELISA |
| Host / Isotype | Human, Rabbit |
| Conjugate | Biotin |
| Physical State | Liquid |
| Purification Method | Recombinant expression, affinity purification IMAC |
| Type | Nanobody |
| Class | Recombinant |
| Affinity (K D ) | K D :Human IgG1: 1.4 nM; Human IgG2: 0.4 nM; Human IgG3: 9.0 nM; Human IgG4: 0.2 nM Rabbit IgG: 0.2 nM Apparent affinity may be higher for full IgGs due to avidity effects (1 antibody captured by 2 Nanobodies). |
| Storage Buffer | 25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % sodium azide |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C/+40°F. Optional: Aliquot upon arrival and store at -20°C/-4°F |
| RRID | AB_2848185 |
| Note | This product is for research use only, not for diagnostic or therapeutic use |
| FH Raphael (Verified Customer) (11-29-2024) | Worked perfectly as expected. Very nice capture signal in SPR and very stable IgG capture (no dissociation at all) thanks to avidity. Applications: Other |
Description
The next level of antibody immobilization to BLI and SPR biosensors • Site-directed and specific immobilization of the Fc-fragment of human IgG and rabbit IgG to streptavidin coated biosensors without biotinylation of the antibody • Compatible to surface plasmon resonance (SPR) and biolayer interferometry (BLI) assays (e.g. FortéBio Octet® systems) • High affinity for stable baseline without significant dissociation • >10x regeneration for multiple reuse • High specificity enables Fc-fragment of human IgG and rabbit IgG antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma samples
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