Nesprin 3 Polyclonal antibody 27132-1-AP proteintech
$149.00
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SKU
27132-1-AP
| Catalog Number | 27132-1-AP |
| Synonyms | C14orf49, Nesprin 3 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF, ELISA |
| Applications | WB, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | C2C12 cells, rat spleen tissue, mouse spleen tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Positive WB detected in | C2C12 cells, rat spleen tissue, mouse spleen tissue |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag26002 Product name: Recombinant human C14orf49 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC146604 Sequence: MTQQPQDDFDRSVEDAQAWMKAVQDQLQVNDNTQGPRAALEARLWETEKICQLEPEGRVRVDLVLRMAEALLACCPGDQKPGILARLKDIKAQWEETVTYMTHCHSRIEWVWLHWSEYLLARDEFYRWFQKMMVTLEPHIELQLGLKEKQWQLSHAQVLLHNVDNQAVLLDRLLEEAASLFNRIGDPSVDEDAQKRMKAEYDAVKAKAQKRVDLLEQVAREHEEYQAGVDEFQLWLKAVVEKVNGCLGRNCKLPITQRLSTLQDIAKDFPRGEESLETLEEQSAGVIRNTSPLGAEKITG Predict reactive species |
| Full Name | chromosome 14 open reading frame 49 |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC146604 |
| Gene Symbol | Nesprin 3 |
| Gene ID (NCBI) | 161176 |
| RRID | AB_2880767 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZMZ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for Nesprin 3 antibody 27132-1-AP | Download protocol |
| WB protocol for Nesprin 3 antibody 27132-1-AP | Download protocol |
| mouse | IF Front Cell Dev Biol SUN5 Interacting With Nesprin3 Plays an Essential Role in Sperm Head-to-Tail Linkage: Research on Sun5 Gene Knockout Mice. Authors - Yunfei Zhang View Article |
| Ning (Verified Customer) (01-28-2023) | Good antibody to prob nesprin 3. Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: mouse cortex lysate Nesprin 3 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:27132-1-AP) |
| Citations | 1 |
| Dilutions | WB : 1:2000-1:10000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718). Protocols Product Specific Protocols IF protocol for Nesprin 3 antibody 27132-1-AP Download protocol WB protocol for Nesprin 3 antibody 27132-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- SUN5 Interacting With Nesprin3 Plays an Essential Role in Sperm Head-to-Tail Linkage: Research on Sun5 Gene Knockout Mice.
Journal: Front Cell Dev Biol | Authors: Yunfei Zhang | Species: mouse | Application: IF
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