PCARE Recombinant monoclonal antibody 83364-4-RR proteintech
$149.00
In stock
SKU
83364-4-RR
| Catalog Number | 83364-4-RR |
| Synonyms | C2orf71, 240352F6 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse retina tissue, mouse eye tissue |
| Tested Applications — Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse eye tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:500-1:2000 |
| Positive WB detected in | mouse retina tissue, mouse eye tissue |
| Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse eye tissue |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:500-1:2000 |
| Tested Reactivity | human, mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag34774 Product name: Recombinant human PCARE protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 10-150 aa of NM_001029883 Sequence: LVNSVAKSGIQFLKKPKAIRPGCQGGSERGSIPLLVKNSTCYDAGEGLAEEQPSPRRNQTTAKGLCQLMGDPASGKRKDMEGLIPGTKTSSSQLNKSQSHMAKDIPFKTQGSHGSQGADFSGDESEESSTQDTSKWKRTAK Predict reactive species |
| Full Name | chromosome 2 open reading frame 71 |
| Calculated Molecular Weight | 140 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | NM_001029883 |
| Gene Symbol | PCARE |
| Gene ID (NCBI) | 388939 |
| RRID | AB_3671018 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| UNIPROT ID | A6NGG8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for PCARE antibody 83364-4-RR | Download protocol |
| IHC protocol for PCARE antibody 83364-4-RR | Download protocol |
| WB protocol for PCARE antibody 83364-4-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:500-1:2000 IF-P : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 240352F6 |
| Conjugation | Unconjugated |
C2orf71/PCARE (photoreceptor cilium actin regulator) is a retina-specific protein consisting of 1289 amino acids with a predicted molecular weight of 140 kDa. PCARE is predicted to be myristoylated and palmitoylated at its N terminus and plays an important role in delivering actin-associated components to the base of the photoreceptor OSs to regulate the initial development of OS disks (PMID: 32312818, 35253837). Protocols Product Specific Protocols IF protocol for PCARE antibody 83364-4-RR Download protocol IHC protocol for PCARE antibody 83364-4-RR Download protocol WB protocol for PCARE antibody 83364-4-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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