| Catalog Number | CL488-10594 |
| Synonyms | NAPP2, Peroxin 14, Peroxin-14, PEX, Pex14p |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | IF/ICC, FC (Intra) |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive IF/ICC detected in | HeLa cells, HepG2 cells |
| Tested Applications — Positive FC (Intra) detected in | HeLa cells |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag0932 Product name: Recombinant human PEX14 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 77-377 aa of BC006327 Sequence: PSSLGPATQVVPVQPPHLISQPYSPAGSRWRDYGALAIIMAGIAFGFHQLYKKYLLPLILGGREDRKQLERMEAGLSELSGSVAQTVTQLQTTLASVQELLIQQQQKIQELAHELAAAKATTSTNWILESQNINELKSEINSLKGLLLNRRQFPPSPSAPKIPSWQIPVKSPSPSSPAAVNHHSSSDISPVSNESTSSSPGKEGHSPEGSTVTYHLLGPQEEGEGVVDVKGQVRMEVQGEEEKREDKEDEEDEEDDDVSHVDEEDCLGVQREDRRGGDGQINEQVEKLRRPEGASNESERD Predict reactive species |
| Full Name | peroxisomal biogenesis factor 14 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC006327 |
| Gene Symbol | PEX14 |
| Gene ID (NCBI) | 5195 |
| RRID | AB_2918988 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75381 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| FC protocol for CL Plus 488 PEX14 antibody CL488-10594 | Download protocol |
| IF protocol for CL Plus 488 PEX14 antibody CL488-10594 | Download protocol |
| mouse | IF J Neuroinflammation TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in septic encephalopathy Authors - Xuliang Huang View Article |
| human | IF Int J Mol Sci Deficiency of PTEN Confers Hypersensitivity to Fatty Acid-Mediated ER Stress in Transformed Hepatocytes. Authors - Olaya Yassin View Article |
| rat | IF Toxicol Appl Pharmacol Ginsenoside Rb1 attenuates hyperoxia-induced lung injury in neonatal rats by inhibiting ferroptosis via the system Xc(-) pathway. Authors - Hiduan Lian View Article |
| Citations | 3 |
| Dilutions | IF/ICC : 1:200-1:800 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | CoraLite® Plus 488 |
PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells. Protocols Product Specific Protocols FC protocol for CL Plus 488 PEX14 antibody CL488-10594 Download protocol IF protocol for CL Plus 488 PEX14 antibody CL488-10594 Download protocol Standard Protocols Click here to view our Standard Protocols
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