| Catalog Number | 11081-1-AP |
| Synonyms | BMIQ4, Dicarboxylate carrier SLC25A8, SLC25A8, UCP 2, UCPH |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (1) |
| Reactivity | human, mouse, rat, pig |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, FC (Intra), ELISA |
| Applications | WB, IHC, FC (Intra), ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse adipose tissue, mouse skeletal muscle tissue, mouse heart tissue, mouse spleen tissue, mouse liver tissue, rat liver tissue |
| Tested Applications — Positive IHC detected in | human cervical cancer tissue, human ovary tissue, human hepatocirrhosis tissue, human skeletal muscle tissue, human liver tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive FC (Intra) detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | mouse adipose tissue, mouse skeletal muscle tissue, mouse heart tissue, mouse spleen tissue, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human cervical cancer tissue, human ovary tissue, human hepatocirrhosis tissue, human skeletal muscle tissue, human liver tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HeLa cells |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag1551 Product name: Recombinant human UCP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-309 aa of BC011737 Sequence: MVGFKATDVPPTATVKFLGAGTAACIADLITFPLDTAKVRLQIQGESQGPVRATASAQYRGVMGTILTMVRTEGPRSLYNGLVAGLQRQMSFASVRIGLYDSVKQFYTKGSEHASIGSRLLAGSTTGALAVAVAQPTDVVKVRFQAQARAGGGRRYQSTVNAYKTIAREEGFRGLWKGTSPNVARNAIVNCAELVTYDLIKDALLKANLMTDDLPCHFTSAFGAGFCTTVIASPVDVVKTRYMNSALGQYSSAGHCALTMLQKEGPRAFYKGFMPSFLRLGSWNVVMFVTYEQLKRALMAACTSREAPF Predict reactive species |
| Full Name | uncoupling protein 2 (mitochondrial, proton carrier) |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 28-30 kDa |
| GenBank Accession Number | BC011737 |
| Gene Symbol | UCP2 |
| Gene ID (NCBI) | 7351 |
| RRID | AB_2213793 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55851 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for UCP2 antibody 11081-1-AP | Download protocol |
| IHC protocol for UCP2 antibody 11081-1-AP | Download protocol |
| IP protocol for UCP2 antibody 11081-1-AP | Download protocol |
| WB protocol for UCP2 antibody 11081-1-AP | Download protocol |
| mouse | WB,IF Sci Rep Genipin inhibits NLRP3 and NLRC4 inflammasome activation via autophagy suppression. Authors - Shui-Xing Yu View Article |
| human | WB EMBO Mol Med Disease-specific phenotypes in iPSC-derived neural stem cells with POLG mutations. Authors - Kristina Xiao Liang View Article |
| rat | WB Metabolism Far-infrared radiation prevents decline in β-cell mass and function in diabetic mice via the mitochondria-mediated Sirtuin1 pathway. Authors - Yung-Ho Hsu View Article |
| Javier (Verified Customer) (07-23-2025) | Excellent antibody for WB Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Mice kidneys |
| David (Verified Customer) (06-03-2022) | Works excellent, I recommend. Applications: Immunofluorescence Primary Antibody Dilution: 1:50 Cell Tissue Type: brain UCP2 Antibody Immunofluorescence validation (1:50 dilution) in brain (Cat no:11081-1-AP) |
| Mohammad (Verified Customer) (06-28-2019) | it is working excellent, I recommend it. Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: mouse hippocampus |
| Citations | 90 |
| Dilutions | WB : 1:500-1:1000 IHC : 1:50-1:500 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
UCP2 (uncoupling protein 2) is a member of the mitochondrial carrier protein super family located in the mitochondrial inner membrane. The UCPs differ greatly in tissue distribution: UCP1 is expressed only in brown adipose; UCP3 preferentially in skeletal muscle, brown adipose and heart; UCP4 and UCP5 mainly in the nervous system (18167556). In contract, UCP2 has a broad distribution and is implicated in a wide range of pathophysiological processes, including diabetes and cancer. This antibody detect a band around 28-33 kDa corresponding to UCP2 protein in mouse liver, skeletal muscle and pancreas. Some upper and lower bands also were observed due to the dimer formation and degradation of the UCP2 protein (20103532). This antibody was raised against the full-length UCP2 protein and may cross-react with UCP3. It is notable that UCP2 is known to have a very short half-life less than 1 hour (17240372). It is strongly recommended that fresh-made lysate be used for western blot assays. Protocols Product Specific Protocols FC protocol for UCP2 antibody 11081-1-AP Download protocol IHC protocol for UCP2 antibody 11081-1-AP Download protocol IP protocol for UCP2 antibody 11081-1-AP Download protocol WB protocol for UCP2 antibody 11081-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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