PLAA Monoclonal antibody 60244-1-Ig proteintech
$149.00
In stock
SKU
60244-1-Ig
| Catalog Number | 60244-1-Ig |
| Synonyms | 5C10G9, DOA1, PLA2P, PLAP |
| Host | Mouse |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | ROS1728 cells, K-562 cells, MCF-7 cells, A431 cells |
| Tested Applications — Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | ROS1728 cells, K-562 cells, MCF-7 cells, A431 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag3224 Product name: Recombinant human PLAA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 496-795 aa of BC032551 Sequence: GAGRYVPGSASMGTTMAGVDPFTGNSAYRSAASKTMNIYFPKKEAVTFDQANPTQILGKLKELNGTAPEEKKLTEDDLILLEKILSLICNSSSEKPTVQQLQILWKAINCPEDIVFPALDILRLSIKHPSVNENFCNEKEGAQFSSHLINLLNPKGKPANQLLALRTFCNCFVGQAGQKLMMSQRESLMSHAIELKSGSNKNIHIALATLALNYSVCFHKDHNIEGKAQCLSLISTILEVVQDLEATFRLLVALGTLISDDSNAVQLAKSLGVDSQIKKYSSVSEPAKVSECCRFILNLL Predict reactive species |
| Full Name | phospholipase A2-activating protein |
| Calculated Molecular Weight | 738 aa, 81 kDa |
| Observed Molecular Weight | 75-80 kDa |
| GenBank Accession Number | BC032551 |
| Gene Symbol | PLAA |
| Gene ID (NCBI) | 9373 |
| RRID | AB_2881366 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9Y263 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for PLAA antibody 60244-1-Ig | Download protocol |
| WB protocol for PLAA antibody 60244-1-Ig | Download protocol |
| Citations | 1 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:50-1:500 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 5C10G9 |
| Conjugation | Unconjugated |
Phospholipase A-2-activating protein (PLAA), also named PLAP, can interact with ubiquitin and is involved in the maintenance of ubiquitin levels. PLAA contains multiple consensus domains including WD repeats, PFU domain and PUL domain. Although the function of PLAA remains unclear, ubiquitin binding of PLAA might be the central role that connects ubiquitination and degradation in ERAD. Recent finding revealed that PLAA served as a novel nociveptive mediator after incision, and its expression level is regulated by miR-203. Protocols Product Specific Protocols IHC protocol for PLAA antibody 60244-1-Ig Download protocol WB protocol for PLAA antibody 60244-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Tumor-derived exosome-based microRNA-206 delivery system as dual modulators of the immune microenvironment and gut microbiota for colorectal cancer therapy
Journal: Int J Biol Macromol | Authors: Jinhua Tian | Application: WB
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