PLAGL2 Monoclonal antibody 68606-1-Ig proteintech
$149.00
In stock
SKU
68606-1-Ig
| Catalog Number | 68606-1-Ig |
| Synonyms | 3C2E5, KIAA0198, Pleiomorphic adenoma-like protein 2, ZNF900 |
| Host | Mouse |
| Reactivity | human, rabbit |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | Caco-2 cells, rabbit testis tissue, COLO 320 cells, HepG2 cells, MOLT-4 cells, Ramos cells, HL-60 cells |
| Tested Applications — Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive FC (Intra) detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | Caco-2 cells, rabbit testis tissue, COLO 320 cells, HepG2 cells, MOLT-4 cells, Ramos cells, HL-60 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, rabbit |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag30227 Product name: Recombinant human PLAGL2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 194-496 aa of BC023655 Sequence: DHCDRRFYTRKDVRRHLVVHTGRKDFLCQYCAQRFGRKDHLTRHVKKSHSQELLKIKTEPVDMLGLLSCSSTVSVKEELSPVLCMASRDVMGTKAFPGMLPMGMYGAHIPTMPSTGVPHSLVHNTLPMGMSYPLESSPISSPAQLPPKYQLGSTSYLPDKLPKVEVDSFLAELPGSLSLSSAEPQPASPQPAAAAALLDEALLAKSPANLSEALCAANVDFSHLLGFLPLNLPPCNPPGATGGLVMGYSQAEAQPLLTTLQAQPQDSPGAGGPLNFGPLHSLPPVFTSGLSSTTLPRFHQAFQ Predict reactive species |
| Full Name | pleiomorphic adenoma gene-like 2 |
| Calculated Molecular Weight | 496 aa, 55 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC023655 |
| Gene Symbol | PLAGL2 |
| Gene ID (NCBI) | 5326 |
| RRID | AB_3085301 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UPG8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for PLAGL2 antibody 68606-1-Ig | Download protocol |
| IHC protocol for PLAGL2 antibody 68606-1-Ig | Download protocol |
| WB protocol for PLAGL2 antibody 68606-1-Ig | Download protocol |
| Citations | - |
| Dilutions | WB : 1:2000-1:10000 IHC : 1:250-1:1000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 3C2E5 |
| Conjugation | Unconjugated |
PLAGL2, also named KIAA0198, belongs to the krueppel C2H2-type zinc-finger protein family. It shows weak transcriptional activatory activity. PLAGL2, a proto-oncogene, is implicated in a variety of cancers including acute myeloid leukemia (AML), malignant glioma, colon cancer, and lung adenocarcinoma. PLAGL2 can function as a tumor suppressor by initiating cell cycle arrest and apoptosis. The calculated molecular weight of PLAGL2 is 55 kDa, but the SUMO1-modifIed form is 68 kDa (PMID: 30535429). Protocols Product Specific Protocols FC protocol for PLAGL2 antibody 68606-1-Ig Download protocol IHC protocol for PLAGL2 antibody 68606-1-Ig Download protocol WB protocol for PLAGL2 antibody 68606-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
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