PLEC Polyclonal antibody 29170-1-AP proteintech
$149.00
In stock
SKU
29170-1-AP
| Catalog Number | 29170-1-AP |
| Synonyms | PLEC1, Plectin, PLEC1b, Plectin 1, PLTN |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, CoIP, ELISA |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, mouse brain tissue, mouse kidney tissue |
| Tested Applications — Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | A549 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:16000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | HEK-293 cells, mouse brain tissue, mouse kidney tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag29418 Product name: Recombinant human PLEC protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1475-1768 aa of NM_201380 Sequence: SETLRRMEEEERLAEQQRAEERERLAEVEAALEKQRQLAEAHAQAKAQAEREAKELQQRMQEEVVRREEAAVDAQQQKRSIQEELQQLRQSSEAEIQAKARQAEAAERSRLRIEEEIRVVRLQLEATERQRGGAEGELQALRARAEEAEAQKRQAQEEAERLRRQVQDESQRKRQAEVELASRVKAEAEAAREKQRALQALEELRLQAEEAERRLRQAEVERARQVQVALETAQRSAEAELQSKRASFAEKTAQLERSLQEEHVAVAQLREEAERRAQQQAEAERAREEAEREL Predict reactive species |
| Full Name | plectin 1, intermediate filament binding protein 500kDa |
| Calculated Molecular Weight | 532 kDa |
| Observed Molecular Weight | 510 kDa |
| GenBank Accession Number | NM_201380 |
| Gene Symbol | Plectin |
| Gene ID (NCBI) | 5339 |
| RRID | AB_3086100 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15149 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for PLEC antibody 29170-1-AP | Download protocol |
| IHC protocol for PLEC antibody 29170-1-AP | Download protocol |
| WB protocol for PLEC antibody 29170-1-AP | Download protocol |
| mouse | IF Int J Gen Med Machine Learning-Derived Diverse Regulated Cell Death Patterns for Therapeutic Target Identification in Glaucoma. Authors - Qianxue Mou View Article |
| human | WB,CoIP Cell Death Dis Epoxy metabolites of linoleic acid promote the development of breast cancer via orchestrating PLEC/NFκB1/CXCL9-mediated tumor growth and metastasis Authors - Kai-Di Ni View Article |
| Marina (Verified Customer) (03-22-2024) | Samples are two tumour (glioblastoma) cell lines (TC1, TC2). Gradient gel 4-15 %. Dilution 1:2000, primary antibody incubation 4ºC overnight, secondary antibody incubation 1 h room temperature. The blot was incubated with anti-plectin antibody after incubation with anti-spectrin beta chain (ref. 67978-1), stripping and re-blocking. The bands appear at the expected size of the target protein plectin (exposure 2 minutes). Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: Human tumour cell lines PLEC Antibody Western Blot validation (1:2000 dilution) in Human tumour cell lines (Cat no:29170-1-AP) |
| Citations | 5 |
| Dilutions | WB : 1:2000-1:16000 IHC : 1:1000-1:4000 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Plectin is a large (≥500-kDa) protein that is normally expressed in various tissues including skin, muscle, and brain. It binds to a number of cytoskeletal proteins including microtubules and intermediate filaments and is involved in establishment and dynamic modulation of the cytoskeletal network. In normal cells, it plays a crucial role in cytoskeleton network organization. In keratinocytes, plectin is concentrated at the basal surface, where it links intermediate filaments to the cytoplasmic domain of transmembrane glycoproteins such as integrin beta-4. (PMID: 24218614, PMID: 28281696, PMID: 23750011) Protocols Product Specific Protocols IF protocol for PLEC antibody 29170-1-AP Download protocol IHC protocol for PLEC antibody 29170-1-AP Download protocol WB protocol for PLEC antibody 29170-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Filamin A in the ovary: a mediator of granulosa cell functions
Journal: Reprod Fertil | Authors: Jiang Yuhao - Machine Learning-Derived Diverse Regulated Cell Death Patterns for Therapeutic Target Identification in Glaucoma.
Journal: Int J Gen Med | Authors: Qianxue Mou | Species: mouse | Application: IF - A disulfidptosis-related gene signature predicts prognosis and immune-metabolic landscape in multiple myeloma.
Journal: Br J Haematol | Authors: Li Wang KO Validated | Species: human | Application: WB - Actomyosin forces trigger a conformational change in desmoplakin within desmosomes.
Journal: Nat Commun | Authors: Yinchen Dong | Species: human | Application: WB,CoIP - Epoxy metabolites of linoleic acid promote the development of breast cancer via orchestrating PLEC/NFκB1/CXCL9-mediated tumor growth and metastasis
Journal: Cell Death Dis | Authors: Kai-Di Ni | Species: human | Application: WB,CoIP
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
