POLE3 Polyclonal antibody 15278-1-AP proteintech
$149.00
In stock
SKU
15278-1-AP
| Catalog Number | 15278-1-AP |
| Synonyms | Arsenic transactivated protein, Arsenic-transactivated protein, AsTP, CHARAC17, CHRAC 17 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, MCF-7 cells, HepG2 cells |
| Tested Applications — Positive IP detected in | HepG2 cells |
| Tested Applications — Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | HeLa cells, MCF-7 cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag7396 Product name: Recombinant human POLE3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-147 aa of BC003166 Sequence: MAERPEDLNLPNAVITRIIKEALPDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTLNASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKTDSEEQDKSRDEDNDEDEERLEEEEQNEEEEVDN Predict reactive species |
| Full Name | polymerase (DNA directed), epsilon 3 (p17 subunit) |
| Calculated Molecular Weight | 17 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC003166 |
| Gene Symbol | POLE3 |
| Gene ID (NCBI) | 54107 |
| RRID | AB_2166751 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRF9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for POLE3 antibody 15278-1-AP | Download protocol |
| IHC protocol for POLE3 antibody 15278-1-AP | Download protocol |
| IP protocol for POLE3 antibody 15278-1-AP | Download protocol |
| WB protocol for POLE3 antibody 15278-1-AP | Download protocol |
| human | IF bioRxiv The nuclear structural protein NuMA is required for efficient DNA replication by ensuring association of core replication factors to replication forks. Authors - Zhen-Guo Wang View Article |
| Citations | 4 |
| Dilutions | WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- SNORA42 enhances prostate cancer cell viability, migration and EMT and is correlated with prostate cancer poor prognosis.
Journal: Int J Biochem Cell Biol | Authors: Chuanyou Yi | Species: human | Application: WB - ASF1B Promotes Oncogenesis in Lung Adenocarcinoma and Other Cancer Types.
Journal: Front Oncol | Authors: Wencheng Zhang | Species: human | Application: WB - The nuclear structural protein NuMA is required for efficient DNA replication and supports the association of core replication factors to replication forks.
Journal: DNA Repair (Amst) | Authors: Zhen-Guo Wang - The nuclear structural protein NuMA is required for efficient DNA replication by ensuring association of core replication factors to replication forks.
Journal: bioRxiv | Authors: Zhen-Guo Wang | Species: human | Application: IF
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