POLR3D Polyclonal antibody 15339-1-AP proteintech
$149.00
In stock
SKU
15339-1-AP
| Catalog Number | 15339-1-AP |
| Synonyms | RPC53, RPC4, DNA-directed RNA polymerase III subunit RPC4, DNA-directed RNA polymerase III subunit D, BN51T |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse brain tissue, mouse testis tissue, HeLa cells, rat brain tissue, rat testis tissue |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | mouse brain tissue, mouse testis tissue, HeLa cells, rat brain tissue, rat testis tissue |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag7542 Product name: Recombinant human POLR3D protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 45-398 aa of BC004484 Sequence: SRDLTLGGVKKKTFTPNIISRKIKEEPKEEVTVKKEKRERDRDRQREGHGRGRGRPEVIQSHSIFEQGPAEMMKKKGNWDKTVDVSDMGPSHIINIKKEKRETDEETKQILRMLEKDDFLDDPGLRNDTRNMPVQLPLAHSGWLFKEENDEPDVKPWLAGPKEEDMEVDIPAVKVKEEPRDEEEEAKMKAPPKAARKTPGLPKDVSVAELLRELSLTKEEELLFLQLPDTLPGQPPTQDIKPIKTEVQGEDGQVVLIKQEKDREAKLAENACTLADLTEGQVGKLLIRKSGRVQLLLGKVTLDVTMGTACSFLQELVSVGLGDSRTGEMTVLGHVKHKLVCSPDFESLLDHKHR Predict reactive species |
| Full Name | polymerase (RNA) III (DNA directed) polypeptide D, 44kDa |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC004484 |
| Gene Symbol | POLR3D |
| Gene ID (NCBI) | 661 |
| RRID | AB_2168013 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05423 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for POLR3D antibody 15339-1-AP | Download protocol |
| IP protocol for POLR3D antibody 15339-1-AP | Download protocol |
| WB protocol for POLR3D antibody 15339-1-AP | Download protocol |
| human | WB FEBS J Stimulation of Pol III-dependent 5S rRNA and U6 snRNA gene expression by AP-1 transcription factors. Authors - Richa Ahuja View Article |
| Citations | 1 |
| Dilutions | WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Polymerase (RNA) III (DNA directed) polypeptide D(POLR3D) plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses and acts as nuclear and cytosolic DNA sensor involved in innate immune response. POLR3D can sense non-self dsDNA that serves as template for transcription into dsRNA. Protocols Product Specific Protocols IF protocol for POLR3D antibody 15339-1-AP Download protocol IP protocol for POLR3D antibody 15339-1-AP Download protocol WB protocol for POLR3D antibody 15339-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Stimulation of Pol III-dependent 5S rRNA and U6 snRNA gene expression by AP-1 transcription factors.
Journal: FEBS J | Authors: Richa Ahuja | Species: human | Application: WB
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