PON1 Polyclonal antibody 18155-1-AP proteintech
$149.00
In stock
SKU
18155-1-AP
| Catalog Number | 18155-1-AP |
| Synonyms | A-esterase 1, Aromatic esterase 1, EC:3.1.1.2, K-45, paraoxonase 1 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | human plasma, mouse liver tissue |
| Tested Applications — Positive IP detected in | mouse liver tissue |
| Tested Applications — Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Positive WB detected in | human plasma, mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag12934 Product name: Recombinant human PON1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-355 aa of BC074719 Sequence: MAKLIALTLLGMGLALFRNHQSSYQTRLNALREVQPVELPNCNLVKGIETGSEDLEILPNGLAFISSGLKYPGIKSFNPNSPGKILLMDLNEEDPTVLELGITGSKFDVSSFNPHGISTFTDEDNAMYLLVVNHPDAKSTVELFKFQEEEKSLLHLKTIRHKLLPNLNDIVAVGPEHFYGTNDHYFLDPYLQSWEMYLGLAWSYVVYYSPSEVRVVAEGFDFANGINISPDGKYVYIAELLAHKIHVYEKHANWTLTPLKSLDFNTLVDNISVDPETGDLWVGCHPNGMKIFFYDSENPPASEVLRIQNILTEEPKVTQVYAENGTVLQGSTVASVYKGKLLIGTVFHKALYCEL Predict reactive species |
| Full Name | paraoxonase 1 |
| Calculated Molecular Weight | 355 aa, 40 kDa |
| Observed Molecular Weight | 35 kDa, 40-45 kDa |
| GenBank Accession Number | BC074719 |
| Gene Symbol | PON1 |
| Gene ID (NCBI) | 5444 |
| RRID | AB_2166315 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P27169 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for PON1 antibody 18155-1-AP | Download protocol |
| IP protocol for PON1 antibody 18155-1-AP | Download protocol |
| WB protocol for PON1 antibody 18155-1-AP | Download protocol |
| human | IHC Sci Rep The role of glucocorticoids in hepatocellular carcinoma through integrated bioinformatics analysis and experimental validation Authors - Ao Wang View Article |
| mouse | WB,IF Biomed Pharmacother GYY4137, a H2S donor, ameliorates kidney injuries in diabetic mice by modifying renal ROS-associated enzymes Authors - Xueqi Chen View Article |
| mouse,human | IHC,ELISA Elife Multi-targeted therapy resistance via drug-induced secretome fucosylation Authors - Mark Borris D Aldonza View Article |
| Citations | 12 |
| Dilutions | WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
PON1, also named PON and K-45, belongs to the paraoxonase family. PON1 hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. It is capable of hydrolyzing a broad spectrum of organophosphate substrates and several aromatic carboxylic acid esters. PON1 may mediate enzymatic protection of low-density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. PON1 was identified as a triple band in a range of approximately 35-40 kDa. For glycosylated, the MW of PON1 is migrated 42-45kd, while the 35 kDa band represents the unglycosylated form (PMID: 17906223, 32002976, 21852972). The antibody has cross-reaction to PON2 and PON3. Protocols Product Specific Protocols IHC protocol for PON1 antibody 18155-1-AP Download protocol IP protocol for PON1 antibody 18155-1-AP Download protocol WB protocol for PON1 antibody 18155-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- GPx1 is involved in the induction of protective autophagy in pancreatic cancer cells in response to glucose deprivation.
Journal: Cell Death Dis | Authors: Qingcai Meng | Species: human | Application: WB - Significance of Liver Zonation in Hepatocellular Carcinoma.
Journal: Front Cell Dev Biol | Authors: Shizhe Yu | Species: human | Application: IF - PGC-1α Inhibits Schwann Cell Dedifferentiation and Delays Peripheral Nerve Degeneration by Targeting PON1
Journal: Cell Mol Neurobiol | Authors: Ying Zou | Species: mouse | Application: WB,IP - GYY4137, a H2S donor, ameliorates kidney injuries in diabetic mice by modifying renal ROS-associated enzymes
Journal: Biomed Pharmacother | Authors: Xueqi Chen | Species: mouse | Application: WB,IF - Multi-targeted therapy resistance via drug-induced secretome fucosylation
Journal: Elife | Authors: Mark Borris D Aldonza | Species: mouse,human | Application: IHC,ELISA - The role of glucocorticoids in hepatocellular carcinoma through integrated bioinformatics analysis and experimental validation
Journal: Sci Rep | Authors: Ao Wang | Species: human | Application: IHC - Significance of Liver Zonation in Hepatocellular Carcinoma.
Journal: Front Cell Dev Biol | Authors: Shizhe Yu | Species: human | Application: IF - The role of glucocorticoids in hepatocellular carcinoma through integrated bioinformatics analysis and experimental validation
Journal: Sci Rep | Authors: Ao Wang | Species: human | Application: IHC - Paraoxonase 1 in endothelial cells impairs vasodilation induced by arachidonic acid lactone metabolite.
Journal: Biochim Biophys Acta Mol Cell Biol Lipids | Authors: Dan Gilad | Species: mouse | Application: IHC - Single nucleotide polymorphism rs854560 in paraoxonase-1 regulates the cytodifferentiation of human periodontal ligament cells
Journal: Front Dent Med | Authors: Risa Masumoto | Species: mouse | Application: IHC - Modulating lysophospholipids with Paraoxonase-1: Exploring its impact on inflammatory responses and immune reactions
Journal: Biochem Biophys Res Commun | Authors: Takuya Shimura | Species: human | Application: WB - Multi-targeted therapy resistance via drug-induced secretome fucosylation
Journal: Elife | Authors: Mark Borris D Aldonza | Species: mouse,human | Application: IHC,ELISA
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