PRAP1 Polyclonal antibody 11932-1-AP proteintech
$149.00
In stock
SKU
11932-1-AP
| Catalog Number | 11932-1-AP |
| Synonyms | Epididymis tissue protein Li 178, PRO1195, proline rich acidic protein 1, Proline-rich acidic protein 1, UNQ608/PRO1195 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | L02 cells, mouse pancreas tissue |
| Tested Applications — Positive IHC detected in | human liver tissue, human kidney tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Positive WB detected in | L02 cells, mouse pancreas tissue |
| Positive IHC detected in | human liver tissue, human kidney tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2526 Product name: Recombinant human PRAP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC029447 Sequence: RLLLVTSLVVVLLWEAGAVPAPKVPIKMQVKHWPSEQDPENRAWGARVVEPPEKDDQLVVLFPVQKPKLLTTEEKPRGQGRGPILPGTKAWMETEDTLGRVLSPEPDHDSLYHPPPEEDQGEERPRLWVMPNHQVLLGPEEDQDHIYHPQ Predict reactive species |
| Full Name | proline-rich acidic protein 1 |
| Calculated Molecular Weight | 151 aa, 17 kDa |
| Observed Molecular Weight | 16-20 kDa |
| GenBank Accession Number | BC029447 |
| Gene Symbol | PRAP1 |
| Gene ID (NCBI) | 118471 |
| RRID | AB_10951856 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96NZ9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for PRAP1 antibody 11932-1-AP | Download protocol |
| WB protocol for PRAP1 antibody 11932-1-AP | Download protocol |
| mouse,human | WB,IF Nat Commun Single-cell RNA sequencing reveals placental response under environmental stress Authors - Eric Van Buren View Article |
| human | IF Elife scRNA-seq and scATAC-seq reveal that Sertoli cell mediates spermatogenesis disorders through stage-specific communications in non-obstructive azoospermia Authors - Shimin Wang View Article |
| Citations | 5 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Proline rich acidic protein 1 (PRAP1) is also named as PRO1195 or UPA. It may play an important role in maintaining normal growth homeostasis in epithelial cells. Mitotic arrest deficient 1 (MAD1) is a key component in mitotic checkpoint signalling. Recent study identified PRAP1 as a novel MAD1 binding partner using yeast-two hybrid screening (PMID: 24374861). PRAP1 has four isoforms with MW 16-20 kDa and 10 kDa. Protocols Product Specific Protocols IHC protocol for PRAP1 antibody 11932-1-AP Download protocol WB protocol for PRAP1 antibody 11932-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Single-cell RNA sequencing reveals placental response under environmental stress
Journal: Nat Commun | Authors: Eric Van Buren | Species: mouse,human | Application: WB,IF - Adipocyte-Derived Exosomal MTTP Suppresses Ferroptosis and Promotes Chemoresistance in Colorectal Cancer
Journal: Adv Sci (Weinh) | Authors: Qiumo Zhang KD Validated | Species: human | Application: WB,IHC - Mesenchymal Stem Cells Carrying Viral Fusogenic Protein p14 to Treat Solid Tumors by Inducing Cell-Cell Fusion and Immune Activation
Journal: Research (Wash D C) | Authors: Yao Wang | Species: human | Application: WB - Dissecting the differentiation origins of intestinal metaplasia and early intestinal-type gastric cancer in gastric antrum by single-cell RNA profiling.
Journal: NPJ Precis Oncol | Authors: Honghao Yin | Species: human | Application: IHC,IF - scRNA-seq and scATAC-seq reveal that Sertoli cell mediates spermatogenesis disorders through stage-specific communications in non-obstructive azoospermia
Journal: Elife | Authors: Shimin Wang | Species: human | Application: IF
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
