PRDM15 Polyclonal antibody 25590-1-AP proteintech
$149.00
In stock
SKU
25590-1-AP
| Catalog Number | 25590-1-AP |
| Synonyms | C21orf83, PFM15, PR domain containing 15, PRDM15, Zinc finger protein 298, ZNF298 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ChIP, ELISA |
| Applications | WB, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | MCF-7 cells, Jurkat cells |
| Tested Applications — Positive IP detected in | MCF-7 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:200-1:1000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Positive WB detected in | MCF-7 cells, Jurkat cells |
| Positive IP detected in | MCF-7 cells |
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag22296 Product name: Recombinant human PRDM15 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 791-1141 aa of BC067102 Sequence: KHCKRHTGIKDFMCELCGKTFSERNTMETHKLIHTVGKQWTCSVCDKKYVTEYMLQKHVQLTHDKVEAQSCQLCGTKVSTRASMSRHMRRKHPEVLAVRIDDLDHLPETTTIDASSIGIVQPELTLEQEDLAEGKHGKAAKRSHKRKQKPEEEAGAPVPEDATFSEYSEKETEFTGSVGDETNSAVQSIQQVVVTLGDPNVTTPSSSVGLTNITVTPITTAAATQFTNLQPVAVGHLTTPERQLQLDNSILTVTFDTVSGSAMLHNRQNDVQIHPQPEASNPQSVAHFINLTTLVNSITPLGSQLSDQHPLTWRAVPQTDVLPPPQPQAPPQQAAQPQVQAEQQQQQMYSY Predict reactive species |
| Full Name | PR domain containing 15 |
| Calculated Molecular Weight | 1507 aa, 169 kDa |
| Observed Molecular Weight | 169 kDa |
| GenBank Accession Number | BC067102 |
| Gene Symbol | PRDM15 |
| Gene ID (NCBI) | 63977 |
| RRID | AB_2880145 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P57071 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IP protocol for PRDM15 antibody 25590-1-AP | Download protocol |
| WB protocol for PRDM15 antibody 25590-1-AP | Download protocol |
| human | WB,IHC,ChIP Biochim Biophys Acta Mol Basis Dis PRDM15 promotes colorectal carcinogenesis by transcriptionally repressing USP10 to destabilize p53. Authors - Chongyang Wu KD Validated View Article |
| Citations | 3 |
| Dilutions | WB : 1:200-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
The PR-domain containing proteins (PRDMs) have a common involvement in the modulation of gene activities. A PR-domain family member usually produces two products, called PR-plus and PR-minus, which differ by the presence or absence of the PR domain, respectively. The PR-plus product is underexpressed or disrupted in cancer cells, whereas the PR-minus product is present or overexpressed in cancer cells. This imbalance in the amount of the two products, which is a result of either genetic or epigenetic events, appears to be a determining factor of malignancy. PRDM15 (PR domain-containing protein 15), also known as PFM15 or ZNF298, is a 1507 amino acid protein belonging to the PRDM family. Localizing to the nucleus, PRDM15 contains sixteen C2H2-type zinc fingers and one SET domain. It is believed to participate in transcriptional regulation and may be involved in cell differentiation and tumorigenesis. PRDM15 exists several isoform and molecular weight of respective isoform is 169 kDa, 135kda, 62kDa. Protocols Product Specific Protocols IP protocol for PRDM15 antibody 25590-1-AP Download protocol WB protocol for PRDM15 antibody 25590-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Single-cell transcriptomics uncovers endothelial progenitor-like remodelling driving human coronary atherosclerosis progression.
Journal: Nat Cell Biol | Authors: Fang Yao - The RNA methyltransferase METTL16 enhances cholangiocarcinoma growth through PRDM15-mediated FGFR4 expression
Journal: J Exp Clin Cancer Res | Authors: Nianli Liu KD Validated | Species: human | Application: WB - PRDM15 promotes colorectal carcinogenesis by transcriptionally repressing USP10 to destabilize p53.
Journal: Biochim Biophys Acta Mol Basis Dis | Authors: Chongyang Wu KD Validated | Species: human | Application: WB,IHC,ChIP
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