PSMB6 Polyclonal antibody 11684-2-AP proteintech
$149.00
In stock
SKU
11684-2-AP
| Catalog Number | 11684-2-AP |
| Synonyms | EC:3.4.25.1, LMPY, Proteasome subunit beta type-6, Y |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, Caco-2 cells, fetal human brain tissue, human liver tissue, mouse brain tissue, rat brain tissue |
| Tested Applications — Positive IHC detected in | human colon cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HeLa cells, Caco-2 cells, fetal human brain tissue, human liver tissue, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human colon cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2296 Product name: Recombinant human PSMB6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-239 aa of BC000835 Sequence: MAATLLAARGAGPAPAWGPEAFTPDWESREVSTGTTIMAVQFDGGVVLGADSRTTTGSYIANRVTDKLTPIHDRIFCCRSGSAADTQAVADAVTYQLGFHSIELNEPPLVHTAASLFKEMCYRYREDLMAGIIIAGWDPQEGGQVYSVPMGGMMVRQSFAIGGSGSSYIYGYVDATYREGMTKEECLQFTANALALAMERDGSSGGVIRLAAIAESGVERQVLLGDQIPKFAVATLPPA Predict reactive species |
| Full Name | proteasome (prosome, macropain) subunit, beta type, 6 |
| Calculated Molecular Weight | 239 aa, 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC000835 |
| Gene Symbol | PSMB6 |
| Gene ID (NCBI) | 5694 |
| RRID | AB_2172199 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P28072 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for PSMB6 antibody 11684-2-AP | Download protocol |
| IHC protocol for PSMB6 antibody 11684-2-AP | Download protocol |
| WB protocol for PSMB6 antibody 11684-2-AP | Download protocol |
| rat | WB Cell Physiol Biochem The Favored Mechanism for Coping with Acute Cold Stress: Upregulation of miR-210 in Rats. Authors - Wenjin Guo View Article |
| mouse | WB Biochem Pharmacol Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP) Authors - Xin Xie View Article |
| human | WB,IF PLoS Genet Nuclear p62/SQSTM1 facilitates ubiquitin-independent proteasomal degradation of BMAL1 Authors - Chenliang Zhang View Article |
| Citations | 7 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:50-1:500 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
PSMB6(Proteasome subunit beta type-6) is also named as LMPY, Y and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It may also catalyzes basal processing of intracellular antigens.It can be up-regulated in anaplastic thyroid cancer cell lines and down-regulated by IFNG/IFN-gamma (at protein level)(PMID:8066462;15613457). Protocols Product Specific Protocols IF protocol for PSMB6 antibody 11684-2-AP Download protocol IHC protocol for PSMB6 antibody 11684-2-AP Download protocol WB protocol for PSMB6 antibody 11684-2-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- The Favored Mechanism for Coping with Acute Cold Stress: Upregulation of miR-210 in Rats.
Journal: Cell Physiol Biochem | Authors: Wenjin Guo | Species: rat | Application: WB - Hepatocyte-specific Wtap deficiency promotes hepatocellular carcinoma by activating GRB2/ERK depending on downregulation of proteasome-related genes
Journal: J Biol Chem | Authors: Xinzhi Li | Species: mouse | Application: WB - Immune regulation and prognostic prediction model establishment and validation of PSMB6 in lung adenocarcinoma
Journal: Front Genet | Authors: Haiyang Zhao | Species: human | Application: WB - Nuclear p62/SQSTM1 facilitates ubiquitin-independent proteasomal degradation of BMAL1
Journal: PLoS Genet | Authors: Chenliang Zhang | Species: human | Application: WB,IF - Downregulation of hepatic METTL3 contributes to APAP-induced liver injury in mice
Journal: JHEP Rep | Authors: Chunhong Liu | Species: mouse | Application: WB - Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP)
Journal: Biochem Pharmacol | Authors: Xin Xie | Species: mouse | Application: WB - The Favored Mechanism for Coping with Acute Cold Stress: Upregulation of miR-210 in Rats.
Journal: Cell Physiol Biochem | Authors: Wenjin Guo | Species: rat | Application: WB
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
