PTPN12 Polyclonal antibody 31215-1-AP proteintech
$149.00
In stock
SKU
31215-1-AP
| Catalog Number | 31215-1-AP |
| Synonyms | PTPG1, PTP PEST |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, MCF-7 cells |
| Tested Applications — Positive IF/ICC detected in | A431 cells, U-251 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, MCF-7 cells |
| Positive IF/ICC detected in | A431 cells, U-251 cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag35382 Product name: Recombinant human PTPN12 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 603-780 aa of NM_002835.3 Sequence: SDDSDSDERNSDGAVTQNKTNISTASATVSAATSTESISTRKVLPMSIARHNIAGTTHSGAEKDVDVSEDSPPPLPERTPESFVLASEHNTPVRSEWSELQSQERSEQKKSEGLITSENEKCDHPAGGIHYEMCIECPPTFSDKREQISENPTEATDIGFGNRCGKPKGPRDPPSEWT Predict reactive species |
| Full Name | protein tyrosine phosphatase, non-receptor type 12 |
| Calculated Molecular Weight | 88kDa |
| Observed Molecular Weight | 110-125 kDa |
| GenBank Accession Number | NM_002835.3 |
| Gene Symbol | PTPN12 |
| Gene ID (NCBI) | 5782 |
| RRID | AB_3669905 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q05209 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for PTPN12 antibody 31215-1-AP | Download protocol |
| WB protocol for PTPN12 antibody 31215-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:1000-1:4000 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
PTPN12 (Tyrosine-protein phosphatase non-receptor type 12), also referred to as PTP-PEST (PTP-proline, glutamic acid, serine, and threonine-rich), is a cytoplasmic PTP expressed in a wide spectrum of cell types. PTPN12 has been identified as a potent tumor suppressor in human cancers and a critical regulator of cell adhesion and migration (PMID: 26391955, 29743287). PTPN12 is phosphorylated on serine and threonine residues and is rich in proline (P), glutamate (E), serine (S), and threonine (T) residues. Western blot analysis detected PTPN12 at an apparent molecular mass of 110 kDa. Protocols Product Specific Protocols IF protocol for PTPN12 antibody 31215-1-AP Download protocol WB protocol for PTPN12 antibody 31215-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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