PTPRS Polyclonal antibody 29415-1-AP proteintech
$149.00
In stock
SKU
29415-1-AP
| Catalog Number | 29415-1-AP |
| Synonyms | Receptor-type tyrosine-protein phosphatase sigma, Receptor-type tyrosine-protein phosphatase S, R PTP sigma, R PTP S, PTPSIGMA |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, A549 cells, HeLa cells |
| Tested Applications — Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:12000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | HEK-293 cells, A549 cells, HeLa cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag30152 Product name: Recombinant human rPTPσ protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 825-1000 aa of NM_002850 Sequence: VVVTKGAVLGRPTLSVQQTPEGSLLARWEPPAGTAEDQVLGYRLQFGREDSTPLATLEFPPSEDRYTASGVHKGATYVFRLAARSRGGLGEEAAEVLSIPEDTPRGHPQILEAAGNASAGTVLLRWLPPVPAERNGAIVKYTVAVREAGALGPARETELPAAAEPGAENALTLQGL Predict reactive species |
| Full Name | protein tyrosine phosphatase, receptor type, S |
| Calculated Molecular Weight | 217 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | NM_002850 |
| Gene Symbol | PTPRS |
| Gene ID (NCBI) | 5802 |
| RRID | AB_2918301 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13332 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for PTPRS antibody 29415-1-AP | Download protocol |
| WB protocol for PTPRS antibody 29415-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:2000-1:12000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
PTPRS, also known as R-PTP-sigma or PTPσ, is a member of the protein tyrosine phosphatase (PTP) family (PMID: 8524829). PTPs dephosphorylate phosphotyrosyl residues in proteins that are phosphorylated by protein-tyrosine kinases (PTKs) (PMID: 20838449). PTPs are essential regulators of signal transduction pathways and play important roles in regulating many cellular processes in conjunction with PTKs (PMID: 7834742). PTPRS contains an extracellular region, a single transmembrane segment and two tandem intracellular catalytic domains, and thus represents a receptor-type PTP. Full-length PTPRS is 217 kDa and has been reported to be proteolytically cleaved into a 140-kDa N-terminal subunit containing extracellular and transmembrane domains and a C-terminal subunit (PMID: 29915291). This antibody raised against 825-1000 aa of human PTPRS detects endogenous PTPRS with an apparent molecular weight of 140 kDa. Protocols Product Specific Protocols IHC protocol for PTPRS antibody 29415-1-AP Download protocol WB protocol for PTPRS antibody 29415-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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