PYGB Polyclonal antibody 12075-1-AP proteintech
$149.00
In stock
SKU
12075-1-AP
| Catalog Number | 12075-1-AP |
| Synonyms | EC:2.4.1.1, GPBB, phosphorylase, glycogen; brain |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | MCF-7 cells, human brain tissue, rat brain tissue, C2C12 cells, fetal human brain tissue, mouse brain tissue |
| Tested Applications — Positive IP detected in | mouse brain tissue |
| Tested Applications — Positive IHC detected in | human stomach cancer tissue, human ovary tumor tissue, mouse heart tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Positive WB detected in | MCF-7 cells, human brain tissue, rat brain tissue, C2C12 cells, fetal human brain tissue, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach cancer tissue, human ovary tumor tissue, mouse heart tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2732 Product name: Recombinant human PYGB protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 544-843 aa of BC017045 Sequence: LKFSAFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHVVTLYNRIKRDPAKAFVPRTVMIGGKAAPGYHMAKLIIKLVTSIGDVVNHDPVVGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGAENLFIFGLRVEDVEALDRKGYNAREYYDHLPELKQAVDQISSGFFSPKEPDCFKDIVNMLMHHDRFKVFADYEAYMQCQAQVDQLYRNPKEWTKKVIRNIACSGKFSSDRTITEYAREIWGVEPSDLQIPPPNIPRD Predict reactive species |
| Full Name | phosphorylase, glycogen; brain |
| Calculated Molecular Weight | 843 aa, 97 kDa |
| Observed Molecular Weight | 97 kDa |
| GenBank Accession Number | BC017045 |
| Gene Symbol | PYGB |
| Gene ID (NCBI) | 5834 |
| RRID | AB_2174885 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11216 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for PYGB antibody 12075-1-AP | Download protocol |
| IP protocol for PYGB antibody 12075-1-AP | Download protocol |
| WB protocol for PYGB antibody 12075-1-AP | Download protocol |
| human | WB Front Cell Dev Biol Glycogen Phosphorylase B Is Regulated by miR101-3p and Promotes Hepatocellular Carcinoma Tumorigenesis. Authors - Guangying Cui View Article |
| mouse | WB J Cell Mol Med PMID: 21251210 Authors - Xi Lei L View Article |
| Patricia (Verified Customer) (02-25-2020) | works fine Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: Ovarian cancer |
| Citations | 24 |
| Dilutions | WB : 1:2000-1:10000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:400-1:1600 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
The three main subtypes of GP found in human tissues-brain-type glycogen phosphorylase (PYGB), liver-type glycogen phosphorylase (PYGL), and muscle-type glycogen phosphorylase (PYGM)-differ in function, structure, and tissue distribution. PYGB (Brain‑type glycogen phosphorylase) is a glycogen phosphorylase and is primarily localized in adult brain tissues and embryo liver tissues, whose function is to provide energy to organisms (PMID: 30106110). PYGB has been observed to be overexpressed in many types of cancer and tumor cell lines and is expected to be a novel target for the diagnosis and treatment of various cancers (PMID: 38334681). Protocols Product Specific Protocols IHC protocol for PYGB antibody 12075-1-AP Download protocol IP protocol for PYGB antibody 12075-1-AP Download protocol WB protocol for PYGB antibody 12075-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.
Journal: Brain | Authors: Scott P Allen | Species: human | Application: WB - PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer
Journal: Int J Biol Sci | Authors: Qian Ji | Species: human | Application: WB - Multi-omics integration reveals the landscape of pro-metastasis metabolism in hepatocellular carcinoma.
Journal: Mol Cell Proteomics | Authors: Yongmei Li | Species: human | Application: WB - Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia
Journal: iScience | Authors: Yu-Han H Hsu - Glycogen Phosphorylase B Is Regulated by miR101-3p and Promotes Hepatocellular Carcinoma Tumorigenesis.
Journal: Front Cell Dev Biol | Authors: Guangying Cui | Species: human | Application: WB - PMID: 21251210
Journal: J Cell Mol Med | Authors: Xi Lei L | Species: mouse | Application: WB - ECD, a novel androgen receptor target promotes prostate cancer tumorigenesis by regulating glycolysis.
Journal: Oncogene | Authors: Mohsin Raza | Species: mouse | Application: IHC - Glycogen and lactate metabolism in mouse fetal Sertoli cells sustain the germ line.
Journal: Cell Rep | Authors: Martín A. Estermann - The airway epithelium undergoes metabolic reprogramming in individuals at high risk for lung cancer.
Journal: JCI Insight | Authors: S M Jamshedur Rahman | Species: human | Application: WB - Multi-omics integration reveals the landscape of pro-metastasis metabolism in hepatocellular carcinoma.
Journal: Mol Cell Proteomics | Authors: Yongmei Li | Species: human | Application: WB - Glycogenolysis-Induced Astrocytic Serping1 Expression Regulates Neuroinflammatory Effects on Hippocampal neuron
Journal: Mol Neurobiol | Authors: Masahito Ishiyama | Species: mouse | Application: WB,IP - Glycogen Phosphorylase B Is Regulated by miR101-3p and Promotes Hepatocellular Carcinoma Tumorigenesis.
Journal: Front Cell Dev Biol | Authors: Guangying Cui | Species: human | Application: WB - Identification of protein targets underlying dietary nitrate-induced protection against doxorubicin cardiotoxicity.
Journal: J Cell Mol Med | Authors: Xi Lei L | Species: mouse | Application: WB - PYGB targeted by androgen receptor contributes to tumor progression and metabolic reprogramming in esophageal squamous carcinoma
Journal: Cell Signal | Authors: Huikai Miao | Species: human | Application: WB,IHC - Epm2b(P71A) and Epm2b(D148N) knock-in mouse models of Lafora disease exhibit distinct and pronounced neurological alterations.
Journal: Prog Neurobiol | Authors: Nerea Iglesias-Cabeza | Species: mouse | Application: WB - Astrocytic glycogenolysis gates Warburg-like metabolic reprogramming that promotes neuropathic pain chronification.
Journal: Prog Neurobiol | Authors: Jun Seo Park | Species: mouse | Application: IHC - Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia
Journal: iScience | Authors: Yu-Han H Hsu - Decreased Glycogenolysis by miR-338-3p Promotes Regional Glycogen Accumulation Within the Spinal Cord of Amyotrophic Lateral Sclerosis Mice.
Journal: Front Mol Neurosci | Authors: Chunyu Li | Species: mouse | Application: WB - The different expression of glycogen phosphorylases in renal clear cell renal carcinoma and chromophobe renal carcinoma
Journal: Clin Proteomics | Authors: Yang Lu | Species: human | Application: WB,IHC - Amyotrophic lateral sclerosis alters the metabolic aging profile in patient derived fibroblasts.
Journal: Neurobiol Aging | Authors: Margarita Gerou | Species: human | Application: WB - Glycogen phosphorylase B promotes ovarian cancer progression via Wnt/β-catenin signaling and is regulated by miR-133a-3p.
Journal: Biomed Pharmacother | Authors: Yang Zhou | Species: human | Application: WB,IHC - Transient down-regulation and restoration of glycogen synthase levels in axotomized rat facial motoneurons.
Journal: Brain Res | Authors: Yosuke Takezawa | Species: rat | Application: WB - Learning-Induced Gene Expression in the Hippocampus Reveals a Role of Neuron -Astrocyte Metabolic Coupling in Long Term Memory.
Journal: PLoS One | Authors: Monika Tadi | Species: mouse | Application: WB - Hyper-Glycosylation as a Central Metabolic Driver of Alzheimer's Disease.
Journal: bioRxiv | Authors: Tara R. Hawkins | Species: mouse | Application: IHC
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