PYGM-Specific Polyclonal antibody 19716-1-AP proteintech
$149.00
In stock
SKU
19716-1-AP
| Catalog Number | 19716-1-AP |
| Synonyms | PYGM, EC:2.4.1.1, Myophosphorylase |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (1) |
| Reactivity | human, mouse, rat, pig |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse heart tissue, rat heart |
| Tested Applications — Positive IHC detected in | rat skeletal muscle tissue, human liver tissue, human skeletal muscle tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | mouse heart tissue, rat heart |
| Positive IHC detected in | rat skeletal muscle tissue, human liver tissue, human skeletal muscle tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | phosphorylase, glycogen, muscle |
| Calculated Molecular Weight | 97 kDa |
| Observed Molecular Weight | 97-105 kDa |
| GenBank Accession Number | NM_005609 |
| Gene Symbol | PYGM |
| Gene ID (NCBI) | 5837 |
| RRID | AB_10642009 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11217 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for PYGM-Specific antibody 19716-1-AP | Download protocol |
| IHC protocol for PYGM-Specific antibody 19716-1-AP | Download protocol |
| WB protocol for PYGM-Specific antibody 19716-1-AP | Download protocol |
| mouse | WB Cells Proteomic Landscape and Deduced Functions of the Cardiac 14-3-3 Protein Interactome Authors - Jia-Hua Qu View Article |
| human | WB medRxiv Identification of disease-specific extracellular vesicle-associated plasma protein biomarkers for Duchenne Muscular Dystrophy and Facioscapulohumeral Muscular Dystrophy Authors - Mustafa Bilal Bayazit View Article |
| Patricia (Verified Customer) (02-25-2020) | Some non-specific bands appeared. Blocked in 5% skim milk and diluted antibody in TBS. Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: Ovarian cancer |
| Citations | 15 |
| Dilutions | WB : 1:2000-1:10000 IHC : 1:50-1:500 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
PYGM belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGM are the cause of glycogen storage disease type 5 (GSD5) which also known as McArdle disease. The antibody is specific to PYGM. Protocols Product Specific Protocols IF protocol for PYGM-Specific antibody 19716-1-AP Download protocol IHC protocol for PYGM-Specific antibody 19716-1-AP Download protocol WB protocol for PYGM-Specific antibody 19716-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Glycogen metabolism links glucose homeostasis to thermogenesis in adipocytes.
Journal: Nature | Authors: Omer Keinan | Species: mouse | Application: WB - PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer
Journal: Int J Biol Sci | Authors: Qian Ji | Species: human | Application: WB - Absence of p.R50X Pygm read-through in McArdle disease cellular models.
Journal: Dis Model Mech | Authors: Guillermo Tarrasó | Species: mouse | Application: WB - Proteomic Landscape and Deduced Functions of the Cardiac 14-3-3 Protein Interactome
Journal: Cells | Authors: Jia-Hua Qu | Species: mouse | Application: WB - Analysis of the expression, function and signaling of glycogen phosphorylase isoforms in hepatocellular carcinoma.
Journal: Oncol Lett | Authors: Lingyu Jiang | Species: human | Application: WB - Identification of disease-specific extracellular vesicle-associated plasma protein biomarkers for Duchenne Muscular Dystrophy and Facioscapulohumeral Muscular Dystrophy
Journal: medRxiv | Authors: Mustafa Bilal Bayazit | Species: human | Application: WB
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