Phospho-MEK1 (Thr386) Polyclonal antibody 28935-1-AP proteintech
$149.00
In stock
SKU
28935-1-AP
| Catalog Number | 28935-1-AP |
| Synonyms | MEK1 (Thr386), p MEK1 , p MEK1 (Thr386), p MEK1 Thr386, Phospho MEK1 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, BSA, Glycerol |
| Applications | WB, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | mitogen-activated protein kinase kinase 1 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC139729 |
| Gene Symbol | MEK1 |
| Gene ID (NCBI) | 5604 |
| ENSEMBL Gene ID | ENSG00000169032 |
| RRID | AB_2918218 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q02750 |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| IF protocol for Phospho-MEK1 (Thr386) antibody 28935-1-AP | Download protocol |
| WB protocol for Phospho-MEK1 (Thr386) antibody 28935-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:1000-1:4000 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis. MEK1 has 2 isoforms produced by alternative splicing with the molecular weight of 43 kDa and 41 kDa. MEK1 may form a heterodimer with MEK2.(PMID:21144847,19219045). Protocols Product Specific Protocols IF protocol for Phospho-MEK1 (Thr386) antibody 28935-1-AP Download protocol WB protocol for Phospho-MEK1 (Thr386) antibody 28935-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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