Phospho-P53 (Ser392) Polyclonal antibody 28963-1-AP proteintech
$149.00
In stock
SKU
28963-1-AP
| Catalog Number | 28963-1-AP |
| Synonyms | p P53, p P53 (Ser392), p P53 Ser392, P53 (Ser392), Phospho P53 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Positive WB detected in | HEK-293 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | tumor protein p53 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC003596 |
| Gene Symbol | P53 |
| Gene ID (NCBI) | 7157 |
| RRID | AB_2918224 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04637 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| WB protocol for Phospho-P53 (Ser392) antibody 28963-1-AP | Download protocol |
| human | WB Chem Biol Interact Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65 Authors - Jingwen Tan View Article |
| Andria (Verified Customer) (01-29-2023) | This antibody detects 2 bands. I believe the correct one is the lower because it is induced upon actinomycin treatment Applications: Western Blot Primary Antibody Dilution: 1:500 Cell Tissue Type: NIH 3T3 Phospho-P53 (Ser392) Antibody Western Blot validation (1:500 dilution) in NIH 3T3 (Cat no:28963-1-AP) |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. Phosphorylated at Ser-315 and Ser-392 by CDK2 in response to DNA-damage.(PMID: 19935675, PMID: 24379683) Protocols Product Specific Protocols WB protocol for Phospho-P53 (Ser392) antibody 28963-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65
Journal: Chem Biol Interact | Authors: Jingwen Tan | Species: human | Application: WB
Product Documents
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
