Phospho-P53 (Ser46) Recombinant monoclonal antibody 80077-9-RR proteintech
$149.00
In stock
SKU
80077-9-RR
| Catalog Number | 80077-9-RR |
| Synonyms | Phospho P53, Phospho P53 (Ser46), Phospho-P53, Phospho-P53(Ser46), Phospho-P53-Ser46 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | etoposide treated HT-29 cells, nocodazole treated A431 cells |
| Tested Applications — Positive IF/ICC detected in | etoposide treated HT-29 cells |
| Tested Applications — Positive FC (Intra) detected in | UV treated A431 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | etoposide treated HT-29 cells, nocodazole treated A431 cells |
| Positive IF/ICC detected in | etoposide treated HT-29 cells |
| Positive FC (Intra) detected in | UV treated A431 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | tumor protein p53 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC003596 |
| Gene Symbol | P53 |
| Gene ID (NCBI) | 7157 |
| RRID | AB_3743329 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P04637 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for Phospho-P53 (Ser46) antibody 80077-9-RR | Download protocol |
| IF protocol for Phospho-P53 (Ser46) antibody 80077-9-RR | Download protocol |
| WB protocol for Phospho-P53 (Ser46) antibody 80077-9-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IF/ICC : 1:250-1:1000 FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 250307A3 |
| Conjugation | Unconjugated |
The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis. Homeodomain-interacting protein kinase 2 (HIPK2) Phosphorylates Ser46 off p53 and p38 can phosphorylate this site. Protocols Product Specific Protocols FC protocol for Phospho-P53 (Ser46) antibody 80077-9-RR Download protocol IF protocol for Phospho-P53 (Ser46) antibody 80077-9-RR Download protocol WB protocol for Phospho-P53 (Ser46) antibody 80077-9-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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