| Catalog Number | 87067-1-RR |
| Synonyms | 2 aminoethanethiol dioxygenase, 2-aminoethanethiol dioxygenase, C10orf22, Cysteamine dioxygenase, EC:1.13.11.19 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, HeLa cells, A-673 cells, PC-3 cells, fetal human brain tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Positive WB detected in | HEK-293 cells, HeLa cells, A-673 cells, PC-3 cells, fetal human brain tissue |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag9598 Product name: Recombinant human ADO protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-270 aa of BC018660 Sequence: MPRDNMASLIQRIARQACLTFRGSWGGRGASDRDAASGAEAPMQPGFPENLSKLKSLLTQLRAEDLNIAPRKATLQPLPPNLPPVTYMHIYETDGFSLGVFLLKSGTSIPLHDHPGMHGMLKVLYGTVRISCMDKLDAGGGQRPRALPPEQQFEPPLQPREREAVRPGVLRSRAEYTEASGPCILTPHRDNLHQIDAVEGPAAFLDILAPPYDPDDGRDCHYYRVLEPVRPKEASSSACDLPREVWLLETPQADDFWCEGEPYPGPKVFP Predict reactive species |
| Full Name | 2-aminoethanethiol (cysteamine) dioxygenase |
| Calculated Molecular Weight | 270 aa, 30 kDa |
| Observed Molecular Weight | 28-30 kDa |
| GenBank Accession Number | BC018660 |
| Gene Symbol | ADO |
| Gene ID (NCBI) | 84890 |
| RRID | AB_3745312 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96SZ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| WB protocol for ADO antibody 87067-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:2000-1:10000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 252126B11 |
| Conjugation | Unconjugated |
ADO, or 2-aminoethanethiol dioxygenase, cysteamine dioxygenase, is a thiol dioxygenase in humans that adds two oxygen atoms to cysteamine, forming hypotaurine (PMID: 39637922). ADO has been reported to exhibit two distinct biological functions with a nonheme iron center. It catalyzes oxidation of both cysteamine in sulfur metabolism and N-terminal cysteine-containing proteins or peptides, such as regulator of G protein signaling 5 (RGS5) (PMID: 34508780). ADO is ubiquitously expressed with the highest levels in brain, heart, and skeletal muscle. Western blot analysis detected two bands at 28~30 kDa as indicated in publications (PMID: 17581819). Protocols Product Specific Protocols WB protocol for ADO antibody 87067-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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