Prolactin Recombinant monoclonal antibody 85042-1-RR proteintech
$149.00
In stock
SKU
85042-1-RR
| Catalog Number | 85042-1-RR |
| Synonyms | 241771A5 |
| Host | Rabbit |
| Reactivity | mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse testis tissue, rat lung tissue, rat spleen tissue |
| Tested Applications — Positive IHC detected in | mouse pituitary gland tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse pituitary gland tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:500-1:2000 |
| Positive WB detected in | mouse testis tissue, rat lung tissue, rat spleen tissue |
| Positive IHC detected in | mouse pituitary gland tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse pituitary gland tissue |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:500-1:2000 |
| Tested Reactivity | mouse, rat |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Eg0937 Product name: recombinant mouse Prolactin protein Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 32-228 aa of NP_035294.2 Sequence: LPICSAGDCQTSLRELFDRVVILSHYIHTLYTDMFIEFDKQYVQDREFMVKVINDCPTSSLATPEDKEQALKVPPEVLLNLILSLVQSSSDPLFQLITGVGGIQEAPEYILSRAKEIEEQNKQLLEGVEKIISQAYPEAKGNGIYFVWSQLPSLQGVDEESKILSLRNTIRCLRRDSHKVDNFLKVLRCQIAHQNNC Predict reactive species |
| Full Name | prolactin |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | NP_035294.2 |
| Gene Symbol | Prl |
| Gene ID (NCBI) | 19109 |
| RRID | AB_3743962 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for Prolactin antibody 85042-1-RR | Download protocol |
| IHC protocol for Prolactin antibody 85042-1-RR | Download protocol |
| WB protocol for Prolactin antibody 85042-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:500-1:2000 IHC : 1:2500-1:10000 IF-P : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 241771A5 |
| Conjugation | Unconjugated |
Prolactin is also named as PRL and belongs to the somatotropin/prolactin family. The proteins encoded by PRL are secreted into the cell surroundings. And they are abundantly expressed in pituitary gland, adenohypophysis, decidua and testis. Indeed, chemically, prolactin appears in a multiplicity of posttranslational forms ranging from size variants to chemical modifications such as phosphorylation or glycosylation. It is not only synthesized in the pituitary gland, as originally described, but also within the central nervous system, the immune system, the uterus and its associated tissues of conception, and even the mammary gland itself (PMID: 11015620). Prolactin acts primarily on the mammary gland by promoting lactation (PMID: 30546056). Protocols Product Specific Protocols IF protocol for Prolactin antibody 85042-1-RR Download protocol IHC protocol for Prolactin antibody 85042-1-RR Download protocol WB protocol for Prolactin antibody 85042-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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