RAB13 Polyclonal antibody 31416-1-AP proteintech
$149.00
In stock
SKU
31416-1-AP
| Catalog Number | 31416-1-AP |
| Synonyms | GIG4, Cell growth inhibiting gene 4 protein, Cell growth-inhibiting gene 4 protein, Ras related protein Rab 13, Ras-related protein Rab-13 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, FC (Intra), ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293T cells, HEK-293 cells, HeLa cells, MCF-7 cells |
| Tested Applications — Positive IHC detected in | mouse testis tissue, mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive FC (Intra) detected in | K-562 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:8000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | HEK-293T cells, HEK-293 cells, HeLa cells, MCF-7 cells |
| Positive IHC detected in | mouse testis tissue, mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | K-562 cells |
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag34898 Product name: Recombinant human RAB13 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 100-200 aa of BC000799 Sequence: QNWMKSIKENASAGVERLLLGNKCDMEAKRKVQKEQADKLAREHGIRFFETSAKSSMNVDEAFSSLARDILLKSGGRRSGNGNKPPSTDLKTCDKKNTNKC Predict reactive species |
| Full Name | RAB13, member RAS oncogene family |
| Calculated Molecular Weight | 203 aa, 23 kDa |
| Observed Molecular Weight | 20-23 kDa |
| GenBank Accession Number | BC000799 |
| Gene Symbol | RAB13 |
| Gene ID (NCBI) | 5872 |
| RRID | AB_3669972 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P51153 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for RAB13 antibody 31416-1-AP | Download protocol |
| IHC protocol for RAB13 antibody 31416-1-AP | Download protocol |
| WB protocol for RAB13 antibody 31416-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:1000-1:8000 IHC : 1:50-1:500 FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063). Protocols Product Specific Protocols FC protocol for RAB13 antibody 31416-1-AP Download protocol IHC protocol for RAB13 antibody 31416-1-AP Download protocol WB protocol for RAB13 antibody 31416-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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