RAB3GAP1 Monoclonal antibody 66688-1-Ig proteintech
$149.00
In stock
SKU
66688-1-Ig
| Catalog Number | 66688-1-Ig |
| Synonyms | 4A3A1, KIAA0066, P130, Rab3 GAP, Rab3 GAP p130 |
| Host | Mouse |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells |
| Tested Applications — Positive IHC detected in | mouse brain tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HEK-293 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells |
| Positive IHC detected in | mouse brain tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag17710 Product name: Recombinant human RAB3GAP1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 502-851 aa of BC146809 Sequence: PGDAGKAGDQLVPDNLKETDKEKGEVGKSWDSWSDSEEEFFECLSDTEELKGNGQESGKKGGPKEMANLRPEGRLYQHGKLTLLHNGEPLYIPVTQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCSLEDFVRWYSPRDYIEEEVIDEKGNVVLKGELSARMKIPSNMWVEAWETAKPIPARRQRRLFDDTREAEKVLHYLAIQKPADLARHLLPCVIHAAVLKVKEEESLENISSVKKIIKQIISHSSKVLHFPNPEDKKLEEIIHQITNVEALIARARSLKAKFGTEKCEQEEEKEDLERFVSCLLEQPEVLVTGAGRGHAGRIIH Predict reactive species |
| Full Name | RAB3 GTPase activating protein subunit 1 (catalytic) |
| Calculated Molecular Weight | 981 aa, 111 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC146809 |
| Gene Symbol | RAB3GAP1 |
| Gene ID (NCBI) | 22930 |
| RRID | AB_2882042 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | Q15042 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for RAB3GAP1 antibody 66688-1-Ig | Download protocol |
| IHC protocol for RAB3GAP1 antibody 66688-1-Ig | Download protocol |
| WB protocol for RAB3GAP1 antibody 66688-1-Ig | Download protocol |
| bing (Verified Customer) (08-04-2021) | very good Ab to probe RAB3GAP1 by WB with the right MW of 130 kd Applications: Western Blot Primary Antibody Dilution: 1:3000 Cell Tissue Type: mouse brain and postmortembrain lysates RAB3GAP1 Antibody Western Blot validation (1:3000 dilution) in mouse brain and postmortembrain lysates (Cat no:66688-1-Ig) |
| Citations | - |
| Dilutions | WB : 1:2000-1:10000 IHC : 1:500-1:2000 IF/ICC : 1:200-1:800 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 4A3A1 |
| Conjugation | Unconjugated |
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