RABL2B Polyclonal antibody 11588-1-AP proteintech
$149.00
In stock
SKU
11588-1-AP
| Catalog Number | 11588-1-AP |
| Synonyms | Rab like protein 2B, Rab-like protein 2B |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | fetal human brain tissue, HepG2 cells, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, mouse brain tissue |
| Tested Applications — Positive IP detected in | fetal human brain tissue |
| Tested Applications — Positive IHC detected in | mouse kidney tissue, human lung cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:3000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | fetal human brain tissue, HepG2 cells, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, mouse brain tissue |
| Positive IP detected in | fetal human brain tissue |
| Positive IHC detected in | mouse kidney tissue, human lung cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2189 Product name: Recombinant human RABL2B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-229 aa of BC024281 Sequence: MAEDKTKPSELDQGKYDADDNVKIICLGDSAVGKSKLMERFLMDGFQPQQLSTYALTLYKHTATVDGRTILVDFWDTAGQERFQSMHASYYHKAHACIMVFDVQRKVTYRNLSTWYTELREFRPEIPCIVVANKIDADINVTQKSFNFAKKFSLPLYFVSAADGTNVVKLFNDAIRLAVSYKQNSQDFMDEIFQELENFSLEQEEEDVPDQEQSSSIETPSEEAASPHS Predict reactive species |
| Full Name | RAB, member of RAS oncogene family-like 2B |
| Calculated Molecular Weight | 229 aa, 26 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC024281 |
| Gene Symbol | RABL2B |
| Gene ID (NCBI) | 11158 |
| RRID | AB_2175954 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UNT1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for RABL2B antibody 11588-1-AP | Download protocol |
| IP protocol for RABL2B antibody 11588-1-AP | Download protocol |
| WB protocol for RABL2B antibody 11588-1-AP | Download protocol |
| human | WB Life Sci Alliance Loss of U11/U12 spliceosome gene ZCRB1 leads to aberrant ciliogenesis and WNT signaling. Authors - Mujeeb Ur Rehman Pirzada View Article |
| Citations | 3 |
| Dilutions | WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Rab-like protein 2B (RABL2B) is a member of a poorly characterised clade of the RAS GTPase superfamily, which plays an essential role in male fertility, sperm intraflagellar transport and tail assembly (PMID: 28553998, PMID: 28138870). A preferential expression of RABL2B in human tissues and lymphoblastoid cell lines was detected which is most pronounced in brain and placenta (PMID: 20138207). Protocols Product Specific Protocols IHC protocol for RABL2B antibody 11588-1-AP Download protocol IP protocol for RABL2B antibody 11588-1-AP Download protocol WB protocol for RABL2B antibody 11588-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- RABL2 positively controls localization of GPCRs in mammalian primary cilia.
Journal: J Cell Sci | Authors: Izumi Dateyama | Species: human | Application: WB,IF - Role of U11/U12 minor spliceosome gene ZCRB1 in Ciliogenesis and WNT Signaling
Journal: bioRxiv | Authors: Geralle Powell-Rodgers | Species: human | Application: WB - Loss of U11/U12 spliceosome gene ZCRB1 leads to aberrant ciliogenesis and WNT signaling.
Journal: Life Sci Alliance | Authors: Mujeeb Ur Rehman Pirzada | Species: human | Application: WB
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