RASEF Polyclonal antibody 11569-1-AP proteintech
$149.00
In stock
SKU
11569-1-AP
| Catalog Number | 11569-1-AP |
| Synonyms | RAB45, Ras and EF-hand domain-containing protein, Ras related protein Rab 45, Ras-related protein Rab-45 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse testis tissue, mouse heart tissue |
| Tested Applications — Positive IP detected in | mouse testis tissue |
| Tested Applications — Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | mouse testis tissue, mouse heart tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2149 Product name: Recombinant human RASEF protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 354-740 aa of BC023566 Sequence: DGLRSALENSYSKFNRSLHINNISPGNTISRSSPKFIGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCFDSGLSTLRDPNEYDSEVEYKHQRGFQRSHGVQESFGGDASDTDVPDIRDEETFGLEDVASVLDWKPQGSVSEGSIVSSSRKPISALSPQTDLVDDNAKSFSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG Predict reactive species |
| Full Name | RAS and EF-hand domain containing |
| Calculated Molecular Weight | 740 aa, 83 kDa |
| Observed Molecular Weight | 83 kDa |
| GenBank Accession Number | BC023566 |
| Gene Symbol | RASEF |
| Gene ID (NCBI) | 158158 |
| RRID | AB_2238112 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IZ41 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for RASEF antibody 11569-1-AP | Download protocol |
| IP protocol for RASEF antibody 11569-1-AP | Download protocol |
| WB protocol for RASEF antibody 11569-1-AP | Download protocol |
| human | IHC Int J Clin Exp Pathol High RASEF expression is associated with a significantly better prognosis in colorectal cancer Authors - Xin Yu View Article |
| Citations | 2 |
| Dilutions | WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
RASEF belongs to the Rab family of small GTPases, which function as molecular switches cycling between GDP-bound (inactive) and GTP-bound (active) states. RASEF is predicted to be involved in regulating membrane traffic and vesicle-mediated transport. Its ability to interact with dynein and dynactin components suggests a specific role in microtubule-based transport processes. Protocols Product Specific Protocols IHC protocol for RASEF antibody 11569-1-AP Download protocol IP protocol for RASEF antibody 11569-1-AP Download protocol WB protocol for RASEF antibody 11569-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- RASEF is a novel diagnostic biomarker and a therapeutic target for lung cancer.
Journal: Mol Cancer Res | Authors: Oshita Hideto H KD Validated | Species: human | Application: WB,IP,IHC,IF - High RASEF expression is associated with a significantly better prognosis in colorectal cancer
Journal: Int J Clin Exp Pathol | Authors: Xin Yu | Species: human | Application: IHC
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