RASEF Polyclonal antibody 28162-1-AP proteintech
$149.00
In stock
SKU
28162-1-AP
| Catalog Number | 28162-1-AP |
| Synonyms | RAB45, Ras and EF-hand domain-containing protein, Ras related protein Rab 45, Ras-related protein Rab-45 |
| Host | Rabbit |
| Reactivity | human, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | LNCaP cells, PC-12 cells |
| Tested Applications — Positive IHC detected in | human stomach tissue, human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:3000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Positive WB detected in | LNCaP cells, PC-12 cells |
| Positive IHC detected in | human stomach tissue, human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Tested Reactivity | human, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag28049 Product name: Recombinant human RASEF protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-245 aa of BC023566 Sequence: MEADGDGEELARLRSVFAACDANRSGRLEREEFRALCTELRVRPADAEAVFQRLDADRDGAITFQEFARGFLGSLRGGRRRDWGPLDPAPAVSEAGPETHDSEEDEGDEDAAAALATSCGPASPGRAWQDFQARLGDEAKFIPREEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHKTRKDEKRKAEEALSDLRRQYETEVGD Predict reactive species |
| Full Name | RAS and EF-hand domain containing |
| Calculated Molecular Weight | 740 aa, 83 kDa |
| Observed Molecular Weight | 90-100 kDa |
| GenBank Accession Number | BC023566 |
| Gene Symbol | RASEF |
| Gene ID (NCBI) | 158158 |
| RRID | AB_2881079 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IZ41 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for RASEF antibody 28162-1-AP | Download protocol |
| WB protocol for RASEF antibody 28162-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:1000-1:3000 IHC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
RASEF, or RAS and EF-hand domain-containing protein, is a member of the Rab GTPase protein family. RASEF is a protein with significant implications in lung cancer, potentially serving as both a prognostic biomarker and a therapeutic target. Its role in cancer cell growth and its interaction with ERK1/2 signaling make it a promising candidate for further research and development of new molecular therapies in lung cancer treatment. Protocols Product Specific Protocols IHC protocol for RASEF antibody 28162-1-AP Download protocol WB protocol for RASEF antibody 28162-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols