RBM17 Recombinant monoclonal antibody 82889-1-RR proteintech
$149.00
In stock
SKU
82889-1-RR
| Catalog Number | 82889-1-RR |
| Synonyms | 230125F6, 45 kDa-splicing factor, RBM 17, RNA-binding motif protein 17, SPF 45 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | MCF-7 cells |
| Tested Applications — Positive IP detected in | MCF-7 cells |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Tested Applications — Positive FC (Intra) detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag0985 Product name: Recombinant human RBM17 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-308 aa of BC007871 Sequence: MSLYDDLGVETSDSKTEGWSKNFKLLQSQLQVKKAALTQAKSQRTKQSTVLAPVIDLKRGGSSDDRQIVDTPPHVAAGLKDPVPSGFSAGEVLIPLADEYDPMFPNDYEKVVKRQREERQRQRELERQKEIEEREKRRKDRHEASGFARRPDPDSDEDEDYERERRKRSMGGAAIAPPTSLVEKDKELPRDFPYEEDSRPRSQSSKAAIPPPVYEEQDRPRSPTGPSNSFLANMGGTVAHKIMQKYGFREGQGLGKHEQGLSTALSVEKTSKRGGKIIVGDATEKDASKKSDSNPLTEILKCPTKVVL Predict reactive species |
| Full Name | RNA binding motif protein 17 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC007871 |
| Gene Symbol | RBM17 |
| Gene ID (NCBI) | 84991 |
| RRID | AB_3670611 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96I25 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for RBM17 antibody 82889-1-RR | Download protocol |
| IF protocol for RBM17 antibody 82889-1-RR | Download protocol |
| IP protocol for RBM17 antibody 82889-1-RR | Download protocol |
| WB protocol for RBM17 antibody 82889-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IF/ICC : 1:500-1:2000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 230125F6 |
| Conjugation | Unconjugated |
RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate. Protocols Product Specific Protocols FC protocol for RBM17 antibody 82889-1-RR Download protocol IF protocol for RBM17 antibody 82889-1-RR Download protocol IP protocol for RBM17 antibody 82889-1-RR Download protocol WB protocol for RBM17 antibody 82889-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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