RECQL4 Polyclonal antibody 17008-1-AP proteintech
$149.00
In stock
SKU
17008-1-AP
| Catalog Number | 17008-1-AP |
| Synonyms | ATP dependent DNA helicase Q4, ATP-dependent DNA helicase Q4, DNA 3'-5' helicase RecQ4, DNA helicase, RecQ-like type 4, EC:5.6.2.4 |
| Host | Rabbit |
| Reactivity | human and More (1) |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HepG2 cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | human gliomas tissue, human colon cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:5000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human gliomas tissue, human colon cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag10707 Product name: Recombinant human RECQL4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 297-652 aa of BC013277 Sequence: CTCTCTRPPSEQEGAVGGERPVPKYPPQEAEQLSHQAAPGPRRVCMGHERALPIQLTVQALDMPEEAIETLLCYLELHPHHWLELLATTYTHCRLNCPGGPAQLQALAHRCPPLAVCLAQQLPEDPGQGSSSVEFDMVKLVDSMGWELASVRRALCQLQWDHEPRTGVRRGTGVLVEFSELAFHLRSPGDLTAEEKDQICDFLYGRVQARERQALARLRRTFQAFHSVAFPSCGPCLEQQDEERSTRLKDLLGRYFEEEEGQEPGGMEDAQGPEPGQARLQDWEDQVRCDIRQFLSLRPEEKFSSRAVARIFHGIGSPCYPAQVYGQDRRFWRKYLHLSFHALVGLATEELLQVAR Predict reactive species |
| Full Name | RecQ protein-like 4 |
| Calculated Molecular Weight | 1208 aa, 133 kDa |
| Observed Molecular Weight | 145-150 kDa |
| GenBank Accession Number | BC013277 |
| Gene Symbol | RECQL4 |
| Gene ID (NCBI) | 9401 |
| RRID | AB_2238324 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94761 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for RECQL4 antibody 17008-1-AP | Download protocol |
| IHC protocol for RECQL4 antibody 17008-1-AP | Download protocol |
| IP protocol for RECQL4 antibody 17008-1-AP | Download protocol |
| WB protocol for RECQL4 antibody 17008-1-AP | Download protocol |
| human | IF bioRxiv USP50 suppresses alternative RecQ helicase use and deleterious DNA2 activity during replication Authors - Hannah L Mackay View Article |
| Citations | 10 |
| Dilutions | WB : 1:1000-1:5000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
RECQ protein-like 4(RECOL4), which has helicase domains contain 7 consensus motifs in the middle, is a gene responsible for Werner syndorme and Bloom syndrome. It forms a stable complex with UBR1 adn UBR2, which has a DNA-stimulated ATPase activity. RECOL4 may involve in repairment of DNA damage by interaction with APE1 and FEN1, key participants in base excision repair. This is a rabbit polyclonal antibody raised against part of C-terminal of RECQ4 of human origin. The calculated molecular weight of EIF3B is 133 kDa, but the modified EIF3B protein is about 145-150 kDa. Protocols Product Specific Protocols IF protocol for RECQL4 antibody 17008-1-AP Download protocol IHC protocol for RECQL4 antibody 17008-1-AP Download protocol IP protocol for RECQL4 antibody 17008-1-AP Download protocol WB protocol for RECQL4 antibody 17008-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation
Journal: Nat Commun | Authors: Xiaohua Xu | Species: human | Application: WB,IP - RECQL4 regulates DNA damage response and redox homeostasis in esophageal cancer.
Journal: Cancer Biol Med | Authors: Guosheng Lyu KD Validated | Species: human | Application: IHC - UBE2O and USP7 co-regulate RECQL4 ubiquitinylation and homologous recombination-mediated DNA repair.
Journal: FASEB J | Authors: Qiuling Huang | Species: human | Application: WB - The Deubiquitinase USP28 Stabilizes Expression of RecQ Family Helicases and Maintains the Viability of Triple Negative Breast Cancer Cells.
Journal: J Biol Chem | Authors: Jiewei Wang | Species: human | Application: WB - PARP1 regulates DNA damage-induced nucleolar-nucleoplasmic shuttling of WRN and XRCC1 in a toxicant and protein-specific manner.
Journal: Sci Rep | Authors: Sebastian Veith | Species: human | Application: IF - USP50 suppresses alternative RecQ helicase use and deleterious DNA2 activity during replication
Journal: bioRxiv | Authors: Hannah L Mackay | Species: human | Application: IF - RecQ protein-like 4 drives cisplatin chemosensitivity of cervical cancer cells by modulating annexin A2
Journal: Drug Dev Res | Authors: Ruixue Wang | Species: human | Application: WB,IHC,IF - The Deubiquitinase USP28 Stabilizes Expression of RecQ Family Helicases and Maintains the Viability of Triple Negative Breast Cancer Cells.
Journal: J Biol Chem | Authors: Jiewei Wang | Species: human | Application: WB - RECQL4 alterations in gliomas and nerve sheath tumors: Expression patterns and therapeutic implications.
Journal: J Neuropathol Exp Neurol | Authors: Sarra Belakhoua | Species: human | Application: WB,IHC - USP50 suppresses alternative RecQ helicase use and deleterious DNA2 activity during replication
Journal: bioRxiv | Authors: Hannah L Mackay | Species: human | Application: IF
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