RGS8 Polyclonal antibody 27394-1-AP proteintech
$149.00
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SKU
27394-1-AP
| Catalog Number | 27394-1-AP |
| Synonyms | Regulator of G-protein signaling 8 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse brain tissue, mouse liver tissue, rat brain tissue |
| Tested Applications — Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse cerebellum tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Positive WB detected in | mouse brain tissue, mouse liver tissue, rat brain tissue |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag26535 Product name: Recombinant human RGS8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 28-198 aa of BC069718 Sequence: NKGMRTRLGCLSHKSDSCSDFTAILPDKPNRALKRLSTEEATRWADSFDVLLSHKYGVAAFRAFLKTEFSEENLEFWLACEEFKKTRSTAKLVSKAHRIFEEFVDVQAPREVNIDFQTREATRKNLQEPSLTCFDQAQGKVHSLMEKDSYPRFLRSKMYLDLLSQSQRRLS Predict reactive species |
| Full Name | regulator of G-protein signaling 8 |
| Calculated Molecular Weight | 198 aa, 23 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC069718 |
| Gene Symbol | RGS8 |
| Gene ID (NCBI) | 85397 |
| RRID | AB_3085953 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P57771 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for RGS8 antibody 27394-1-AP | Download protocol |
| IHC protocol for RGS8 antibody 27394-1-AP | Download protocol |
| WB protocol for RGS8 antibody 27394-1-AP | Download protocol |
| mouse | IF Cell Death Discov FGF13 prevents age-related hearing loss by protecting spiral ganglion neurons and ribbon synapses from injury Authors - Huan Yin View Article |
| Citations | 1 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:50-1:500 IF-P : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
RGS8 is a member of the regulator of G protein signaling (RGS) family and encodes a protein with a single RGS domain. Regulator of G protein signaling (RGS) proteins are regulatory and structural components of G protein-coupled receptor complexes. They accelerate transit through the cycle of GTP binding and hydrolysis to GDP, thereby terminating signal transduction, but paradoxically, also accelerate receptor-stimulated activation. Protocols Product Specific Protocols IF protocol for RGS8 antibody 27394-1-AP Download protocol IHC protocol for RGS8 antibody 27394-1-AP Download protocol WB protocol for RGS8 antibody 27394-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- FGF13 prevents age-related hearing loss by protecting spiral ganglion neurons and ribbon synapses from injury
Journal: Cell Death Discov | Authors: Huan Yin | Species: mouse | Application: IF
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