RHOF Polyclonal antibody 12290-1-AP proteintech
$149.00
In stock
SKU
12290-1-AP
| Catalog Number | 12290-1-AP |
| Synonyms | ARHF, Rho family GTPase Rif, Rho in filopodia, RHOF, RIF |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IP, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A549 cells, HeLa cells, human brain tissue, COLO 320 cells, mouse brain tissue, rat brain tissue |
| Tested Applications — Positive IP detected in | COLO 320 cells |
| Tested Applications — Positive IHC detected in | human colon cancer tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Positive WB detected in | A549 cells, HeLa cells, human brain tissue, COLO 320 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human colon cancer tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2943 Product name: Recombinant human RHOF protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-170 aa of BC018208 Sequence: MDAPGALAQTAAPGPGRKELKIVIVGDGGCGKTSLLMVYSQGSFPEHYAPSVFEKYTASVTVGSKEVTLNLYDTAGQEDYDRLRPLSYQNTHLVLICYDVMNPTSYDNVLIKWFPEVTHFCRGIPMVLIGCKTDLRKDKEQLRKLRAAQLEPITYMQVGRGQDPGAQPWL Predict reactive species |
| Full Name | ras homolog gene family, member F (in filopodia) |
| Calculated Molecular Weight | 211 aa, 24 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC018208 |
| Gene Symbol | RHOF |
| Gene ID (NCBI) | 54509 |
| RRID | AB_10644126 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HBH0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for RHOF antibody 12290-1-AP | Download protocol |
| IP protocol for RHOF antibody 12290-1-AP | Download protocol |
| WB protocol for RHOF antibody 12290-1-AP | Download protocol |
| human | WB Cell Biosci SIAH2-EPHB6 axis enhances filopodia formation in hepatocellular carcinoma cells by regulating RHOF. Authors - Qinghe Hu View Article |
| human,mouse | WB,IHC,IF iScience RHOF activation of AKT/β-catenin signaling pathway drives acute myeloid leukemia progression and chemotherapy resistance Authors - Xin Wen KD Validated View Article |
| Citations | 6 |
| Dilutions | WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- Rho family small GTPase Rif regulates Wnt5a-Ror1-Dvl2 signaling and promotes lung adenocarcinoma progression
Journal: J Biol Chem | Authors: Michiru Nishita | Species: human | Application: WB - Deciphering key roles of B cells in prognostication and tailored therapeutic strategies for lung adenocarcinoma: a multi-omics and machine learning approach towards predictive, preventive, and personalized treatment strategies
Journal: EPMA J | Authors: Jinjin Zhang | Species: human | Application: WB - RHOF activation of AKT/β-catenin signaling pathway drives acute myeloid leukemia progression and chemotherapy resistance
Journal: iScience | Authors: Xin Wen KD Validated | Species: human,mouse | Application: WB,IHC,IF - An Integrated Multi-Omics Analysis Identifying Immune Subtypes of Pancreatic Cancer
Journal: Int J Mol Sci | Authors: Yongcheng Su | Species: human | Application: WB - High expression of RHOF is an effective diagnostic marker and a potential prognostic indicator for primary mediastinal large B-cell lymphoma
Journal: Virchows Arch | Authors: Xinyi Zhu | Species: human | Application: IHC - SIAH2-EPHB6 axis enhances filopodia formation in hepatocellular carcinoma cells by regulating RHOF.
Journal: Cell Biosci | Authors: Qinghe Hu | Species: human | Application: WB
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