RNF217 Polyclonal antibody 26084-1-AP proteintech
$149.00
In stock
SKU
26084-1-AP
| Catalog Number | 26084-1-AP |
| Synonyms | C6orf172, IBRDC1, ring finger protein 217, RNF217 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag22022 Product name: Recombinant human RNF217 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-275 aa of BC026087 Sequence: MKVQLGQVEIKCPITECFEFLEETTVVYNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESKYKIQCPTCQFVWCFKCHSPWHEGVNCKEYKKGDKLLRHWASEIEHGQRNAQKCPKCKIHIQRTEGCDHMTCSQCNTNFCYRCGERYRQLRFFGDHTSNLSIFGCKYRYLPERPHLRRLVRGSVCAGKLFIAPLIMVLGLALGAIAVVIVEEIKTYWNLISGRTRNQTQHLAPQPVLLSDMLYCLKQVFICISYLLPL Predict reactive species |
| Full Name | ring finger protein 217 |
| Calculated Molecular Weight | 275 aa, 32 kDa |
| Observed Molecular Weight | 59, 32 kDa |
| GenBank Accession Number | BC026087 |
| Gene Symbol | RNF217 |
| Gene ID (NCBI) | 154214 |
| RRID | AB_3085839 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TC41 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for RNF217 antibody 26084-1-AP | Download protocol |
| IHC protocol for RNF217 antibody 26084-1-AP | Download protocol |
| Citations | - |
| Dilutions | IHC : 1:50-1:500 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
E3 ubiquitin-protein ligase RNF217, also known as IBR domain-containing protein 1 (IBRDC1), as a transmembrane (TM) domain-containing RBR-type E3 ubiquitin-protein ligase, belongs to a highly conserved member of the RING finger family. RNF217 mediates the degradation of the iron exporter ferroportin/SLC40A1 and thus regulates iron homeostasis(Uniprot, PMID: 33895792). Protocols Product Specific Protocols IF protocol for RNF217 antibody 26084-1-AP Download protocol IHC protocol for RNF217 antibody 26084-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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