RNF4 Recombinant monoclonal antibody 84770-1-RR proteintech
$149.00
In stock
SKU
84770-1-RR
| Catalog Number | 84770-1-RR |
| Synonyms | 242197A10, E3 ubiquitin ligase RNF4, E3 ubiquitin-protein ligase RNF4, EC:2.3.2.27, Protein SNURF |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Tested Applications — Positive FC (Intra) detected in | MCF-7 cells |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag12115 Product name: Recombinant human RNF4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-190 aa of BC031935 Sequence: MSTRKRRGGAINSRQAQKRTREATSTPEISLEAEPIELVETAGDEIVDLTCESLEPVVVDLTHNDSVVIVDERRRPRRNARRLPQDHADSCVVSSDDEELSRDRDVYVTTHTPRNARDEGATGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI Predict reactive species |
| Full Name | ring finger protein 4 |
| Calculated Molecular Weight | 190 aa, 21 kDa |
| GenBank Accession Number | BC031935 |
| Gene Symbol | RNF4 |
| Gene ID (NCBI) | 6047 |
| RRID | AB_3743718 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P78317 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for RNF4 antibody 84770-1-RR | Download protocol |
| IF protocol for RNF4 antibody 84770-1-RR | Download protocol |
| Citations | - |
| Dilutions | IF/ICC : 1:50-1:500 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 242197A10 |
| Conjugation | Unconjugated |
RNF4 is a E3 ubiquitin-protein ligase that binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. It regulates the degradation of several proteins including PML and PEA3[PMID:18408734]. It also participates in chromosome alignment and spindle assembly, and regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. In addition, RNF4 can regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 [PMID:20026589]. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation [PMID:11319220]. Protocols Product Specific Protocols FC protocol for RNF4 antibody 84770-1-RR Download protocol IF protocol for RNF4 antibody 84770-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
