ROD1 Polyclonal antibody 14027-1-AP proteintech
$149.00
In stock
SKU
14027-1-AP
| Catalog Number | 14027-1-AP |
| Synonyms | PTBP3, Regulator of differentiation 1, ROD1 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, CoIP, RIP, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HL-60 cells, mouse thymus tissue, HeLa cells, Jurkat cells, rat thymus tissue |
| Tested Applications — Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:12000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Positive WB detected in | HL-60 cells, mouse thymus tissue, HeLa cells, Jurkat cells, rat thymus tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag5172 Product name: Recombinant human ROD1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC044585 Sequence: MDASPSPFSLPKKLNELSARRGSDELLSSGIINGPFTMNSSTPSTANGNDSKKFKRDRPPCSPSRVLHLRKIPCDVTEAEIISLGLPFGKVTNLLMLKGKSQAFLEMASEEAAVTMVNYYTPITPHLRSQPVYIQYSNHRELKTDNLPNQARAQAALQAVSAVQSGSLALSGGPSNEGTVLPGQSPVLRIIIENLFYPVTLEVLHQIFSKFGTVLKIITFTKNNQFQALLQYADPVNAHYAKMALDGQNIYNACCTLRIDFSKLTSLNVKYNNDKSRDFTRLDLPTGDGQPSLEPPMAAAFGAPGIISSPYAGAAGFAPAIGFPQATGLSVPAVPGALGPLTITSSAVTG Predict reactive species |
| Full Name | ROD1 regulator of differentiation 1 (S. pombe) |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 55-59 kDa |
| GenBank Accession Number | BC044585 |
| Gene Symbol | ROD1 |
| Gene ID (NCBI) | 9991 |
| RRID | AB_2182321 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95758 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for ROD1 antibody 14027-1-AP | Download protocol |
| WB protocol for ROD1 antibody 14027-1-AP | Download protocol |
| human,mouse | WB,CoIP Cell Res The RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes. Authors - Juan Chen KO Validated View Article |
| human | WB Oncol Lett Overexpression of ROD1 inhibits invasion of breast cancer cells by suppressing the translocation of β-catenin into the nucleus. Authors - Ya Zhou View Article |
| mouse | WB Mol Cell PTBP1-activated co-transcriptional splicing controls epigenetic status of pluripotent stem cells Authors - Camilla Iannone View Article |
| Annabel (Verified Customer) (12-23-2025) | Produces a single clear band with a strong signal by western blot. Imaged using blotting accelerator Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: B Cells ROD1 Antibody Western Blot validation (1:1000 dilution) in B Cells (Cat no:14027-1-AP) |
| Yaroslav (Verified Customer) (08-03-2022) | multiple bands detected at all tested dulitions (1:3000-1:10000) Applications: Western Blot Primary Antibody Dilution: 1:5000 Cell Tissue Type: mouse embryonic stem cells ROD1 Antibody Western Blot validation (1:5000 dilution) in mouse embryonic stem cells (Cat no:14027-1-AP) |
| Citations | 10 |
| Dilutions | WB : 1:2000-1:12000 IHC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
ROD1 is a RNA-binding protein that mediates pre-mRNA alternative splicing regulation, it plays a role in the regulation of cell proliferation, differentiation and migration. ROD1 exist some isoforms and also can be modified with the molecular weight range from 55-59 kDa. Protocols Product Specific Protocols IHC protocol for ROD1 antibody 14027-1-AP Download protocol WB protocol for ROD1 antibody 14027-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- The RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes.
Journal: Cell Res | Authors: Juan Chen KO Validated | Species: human,mouse | Application: WB,CoIP - Polypyrimidine tract-binding protein 3/insulin-like growth factor 2 mRNA-binding proteins 3/high-mobility group A1 axis promotes renal cancer growth and metastasis
Journal: iScience | Authors: Qianqing Wang - PTBP3 regulates proliferation of lung squamous cell carcinoma cells via CDC25A-mediated cell cycle progression.
Journal: Cancer Cell Int | Authors: Yingji Chen | Species: human | Application: WB - PTBP3 promotes migration of non-small cell lung cancer through regulating E-cadherin in EMT signaling pathway.
Journal: Cancer Cell Int | Authors: Qiong Wu | Species: human | Application: WB,IHC - Overexpression of ROD1 inhibits invasion of breast cancer cells by suppressing the translocation of β-catenin into the nucleus.
Journal: Oncol Lett | Authors: Ya Zhou | Species: human | Application: WB - PTBP1-activated co-transcriptional splicing controls epigenetic status of pluripotent stem cells
Journal: Mol Cell | Authors: Camilla Iannone | Species: mouse | Application: WB - PTBP3 regulates proliferation of lung squamous cell carcinoma cells via CDC25A-mediated cell cycle progression.
Journal: Cancer Cell Int | Authors: Yingji Chen | Species: human | Application: WB - CircCRIM1 promotes the translation of HIF-1α protein through its interaction with PTBP3 and enhances malignant progression and angiogenesis in glioblastoma
Journal: Biochem Pharmacol | Authors: Yating Liu | Species: human | Application: WB - Polypyrimidine tract-binding protein 3/insulin-like growth factor 2 mRNA-binding proteins 3/high-mobility group A1 axis promotes renal cancer growth and metastasis
Journal: iScience | Authors: Qianqing Wang - Overexpression of ROD1 inhibits invasion of breast cancer cells by suppressing the translocation of β-catenin into the nucleus.
Journal: Oncol Lett | Authors: Ya Zhou | Species: human | Application: WB
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